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Your Position: Casa > Kits > Organoids > RIPO-IWM006

Human iPSC-Derived Intestinal Organoid Maintenance Kit

For research use only.

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Materials Provided
Box A(2-8°C)
IDComponentsSize
RIPO-IWM006-C01Intestinal organoid Basal medium MM225ml
 
Box B(-20°C)
IDComponentsSize
RIPO-IWM006-1-C01Intestinal organoid Supplement MM25ml
  • Product Details
    Intestinal organoids are three-dimensional in vitro models with a cellular composition and structural organization that is representative to the human Intestinal regions. Human iPSC-Derived Intestinal Organoid Maintenance Kit allows long-term maturation and maintenance of the Intestinal organoids.
  • Product Specification
    The basic medium of this kit is a serum-free, well-defined medium with minimal batch variation to which differentiation factors are added. This medium does not contain antibiotics, the addition of which may affect organoid differentiation.
  • Storage

    The unopened Box A is stable for 12 months from the date of manufacture if stored under 2-8°C.
    The unopened Box B is stable for 12 months from the date of manufacture if stored under -20°C.
    The opened kit should be stored according to components table.

*Please note that to support full process of organoid culture and maintenance, this product have to be used with Human iPSC-Derived Intestinal Organoid Maintenance Kit (Cat. No.RIPO-IWM006),you can follow this link for product information.
Product Diagram
 Organoids PRODUCT DIAGRAM

Protocol Diagram of Intestinal organoid differentiation.

Validation Data
Organoid Histology and State
 Organoids Organoid Histology And State

The Intestinal organoids differentiated using the Human iPSC-Derived Intestinal Organoid Differentiation Kit (Ca. No. RIPO-IWM005K) show regular peristalsis.

Orgnaoid Histology
 Organoids ORGNAOID HISTOLOGY

Observation of granulocyte like cells, adipocyte like cells, smooth muscle like cells and intestinal crypt like structure by morphology on day 120 Intestinal organoids.

Marker Expression
 Organoids MARKER EXPRESSION

The Intestinal organoids differentiated using the Human iPSC-Derived Intestinal Organoid Differentiation Kit (Ca. No. RIPO-IWM005K) show expression of enterochromaffin cells (CHGA) and endothelial cells (CD31).

 Organoids MARKER EXPRESSION

The Intestinal organoids differentiated using the Human iPSC-Derived Intestinal Organoid Differentiation Kit (Ca. No. RIPO-IWM005K) show expression of goblet cells (mucus-producing, MUC2) and endothelial cells (CD31).

 Organoids MARKER EXPRESSION

The Intestinal organoids differentiated using the Human iPSC-Derived Intestinal Organoid Differentiation Kit (Ca. No. RIPO-IWM005K) show expression of smooth muscle cell (α-SMA); epithelial cell (EPCAM); macrophage (CD68); goblet cell (MUC2); Ki67 (intestinal stem cell) and enteroendocrine cells (CHGA).

Organoid Activity
 Organoids ORGANOID ACTIVITY

Intestinal organoids differentiated using the Human iPSC-Derived Intestinal Organoid Differentiation Kit (Ca. No. RIPO-IWM005K) show normal intestinal function validated by the absorption of fatty acid and glucose.

  • Clinical and Translational Updates

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Price(EUR) : €190.00

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