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| Assay Type | Inhibition-TR-FRET |
| Analyte | Human IgG, Human IgG Fc protein, Human FcRn inhibitors, Anti-human FcRn antibody |
| Format | 100T/500T |
| Reactivity | Human |
| Regulatory Status | RUO |
| Sensitivity | IC50=20.14μg/mL |
| Standard Curve Range | 0.24 μg/mL-1000 μg/mL |
| Assay Time | 1 hr |
| Suitable Sample Type | For the binding of IgG Fc region to the human FcRn receptor |
| Sample volume | 10 μL |
The Human FcRn Binding Kit (TR-FRET) takes advantage of binding of Europium-chelate labeled FcRn (donor) and FA labeled Human IgG1 antibody (acceptor) in a homogeneous (no wash) TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) competition assay to measure the interaction between human FcRn and antibody drug candidates or FcRn inhibitors. It is designed to facilitate the half-life evaluation of antibody drug candidates, and also high-throughput screening of FcRn inhibitors within 0.5-1 hours. It is highly sensitive, has a short detection time and easy to use.
This kit is designed to facilitate the half-life evaluation of antibody drug candidates, and also high-throughput screening of FcRn inhibitors. It can also be used as a universal detection tool to identifying the ability of antibody drugs to bind to FcRn.
It is for research use only.
2. Find the expiration date on the outside packaging and do not use reagents past their expiration date.
3. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
| ID | Components | Size |
| FRT01-C01 | Human FCRN&B2M Heterodimer Protein Europium-chelate | 2.7 μg/100 tests 13.5 μg/500 tests |
| FRT01-C02 | FA labeled human IgG antibody | 2.4 μg/100 tests 12 μg/500 tests |
| FRT01-C03 | Human IgG standard | 200 μg/100 tests 1 mg/500 tests |
| FRT01-C04 | Sample Dilution Buffer | 10 mL/100tests & 500tests |
| FRT01-C05 | Detection Buffer | 10 mL/100tests & 500tests |
Inhibition Assay of interaction of Human FcRn and Human IgG1 antibody by Human IgG standard in a homogeneous (no wash) TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) competition assay, with a typical IC50 of 20.14 μg/mL (QC tested).
The kit has been used to detect different subclasses of Human IgG Fc proteins, which exhibit different IC50 results as expected.
The kit has been used to detect different subclasses of Human IgG, which exhibit different IC50 results as expected.
The kit has been used to detect the binding activity between Human FcRn and Human IgG standard under different pH. The IC50 shows a significant increase with increasing pH, which corelates with a decreased binding between Human FcRn and Human IgG standard.
The half-lives of these 3 monoclonal antibodies currently in clinical use generally correlate with the binding affinity to FcRn. The kit has been used to detect 3 FDA approved antibody drugs of different binding affinity to FcRn, and the IC50 trends are consistent with affinity constant from SPR as well as the actual in vivo half-life published.
The kit is suitable for the detection of FcRn inhibitors. It shows that both Efgartigimod and its biosimilar, Human IgG1 Fc (C103S, M135Y, S137T, T139E, H316K, N317F) His Tag (Cat. No. IG1-H52H8) exhibit good inhibitory activity in this TR-FRET competition assay.
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