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ID | Components | Size |
RAB001-C01 | High-bind Plate | 1 plate |
RAB001-C02 | Mouse FMC63 scFv | 25 μg |
RAB001-C03 | FMC63 ADA Standard | 15 μg |
RAB001-C04 | Biotin-Mouse FMC63 scFv | 20 μg |
RAB001-C05 | Streptavidin-HRP | 50 μL |
RAB001-C06 | Coating Buffer | 12 mL |
RAB001-C07 | 10xWashing Buffer | 50 mL |
RAB001-C08 | Blocking Buffer | 50 mL |
RAB001-C09 | Substrate Solution | 12 mL |
RAB001-C10 | Stop Solution | 7 mL |
This kit is developed for detection of Anti-CD19 (FMC63) antibodies in CAR Immunogenicity assay test. Detection and monitoring the level of ADA is important to fully understand the potential immune responses.
It is for research use only.
The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
The kit shipped at room temperature that had been validated. Please contact us if you need blue ice shipping, but additional freight may be followed.
Below is the Shipping Statement for Kit Products.
Your experiment will include 4 simple steps:
a) Coat the plate with Mouse FMC63 scFv.
b) Add your molecule of interest to the tests.
c) Add Mouse FMC63 scFv-Biotin.
d) Add Streptavidin-HRP followed by TMB or other colorimetric HRP substrate.
Detection of FMC63 ADA titer by bridging-ELISA Assay.
Immobilized Mouse FMC63 scFv can bind FMC63 ADA in 1:20 human serum, and then add Biotin-Mouse FMC63 scFv. Detection was performed using HRP-conjugated streptavidin with sensitivity of 19 ng/mL (QC tested).
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
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