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> Servicio de ensayo inmunoenzimático (ELISA)
Los ensayos inmunoenzimáticos (ELISA) llevan décadas siendo uno de los principales métodos de detección de analitos. Como experimento fundamental de cualquier investigación de desarrollo de fármacos, los datos ELISA fiables y de alta calidad son críticos para la comprensión científica y las investigaciones posteriores.
La ventaja de ACRO radica en nuestro personal científico cualificado y experimentado, nuestro amplio catálogo de proteínas de producción propia, nuestros anticuerpos, incluidos los anticuerpos antiidiotipos, y nuestros kits ELISA listos para usar.
Ofrecemos un servicio integral de desarrollo de métodos de bioensayo ELISA, que incluye el desarrollo de métodos, la validación, la transferencia y el desarrollo de kits, de acuerdo con cada una de sus necesidades para ayudarle a acelerar el proceso de desarrollo de fármacos!
ACROBiosystems proporciona mediciones de absorbancia, fluorescencia y luminiscencia de alta calidad empleando el sistema SpectraMax i3x (Molecular Devices). ACROBiosystems también ha adquirido la expansión ScanLaterTM Western Blot así como el cartucho de detección Alpha Screen 384 STD proporcionando a nuestros clientes múltiples opciones de análisis.
Sensibilidad en todo el espectro con iluminación Spectral Fusion™
Rango dinámico ampliado con PMT refrigerado
Cartuchos para FP, AlphaScreen y Western Imaging
Basándose en el sistema SpectraMax i3x, ACROBiosystems presta servicios de investigación y desarrollo biomédicos a clientes que buscan la cuantificación y detección de la interacción anticuerpo-antígeno. Otros servicios incluyen, entre otros, la actividad antidroga (ADA), ensayos PK/PD y análisis de anticuerpos.
El informe analítico se entregará en 2 semanas tras la recepción de la muestra!
ACROBiosystems ofrece una amplia gama de servicios de desarrollo de métodos de bioensayo ELISA, desde los reactivos de ensayo hasta la detección de muestras!
Servicio integral de desarrollo de métodos de bioensayo ELISA
We provide screening services including peptide screening and antibody screening using common methods like competitive ELISA.
For example, we can perform a screening assay for peptide samples using ACROBiosystem's own Anti-SARS-CoV-2 Neutralizing Antibody Titer Serologic Kit (Cat#: RAS-N022).
The microplate in the kit has been pre-coated with Human ACE2 protein. First serum samples, Positive control, Negative Control are added to the wells followed by addition of HRP-SARS-CoV-2 Spike RBD. The presence of neutralizing antibodies in samples will compete with ACE2 for HRP-SARS-CoV-2 Spike RBD binding. The intensity of assay signal decrease proportionally to the presence of Anti-SARS-CoV-2 neutralizing antibody.
Fig1. Positive reference for anti-SARS-CoV-2 neutralizing antibody concentrations (μg/mL) for peptide 9 screening (SpectraMax i3x).
Fig2. Sample screening for peptide 9 (uM) using anti-SARS-CoV-2 neutralizing antibody titer serologic Kit (Cat#: RAS-N022) (SpectraMax i3x).
Fig3. Different anti-BCMA antibodies have different neutralization ability for BCMA and BAFF.
ln addition to antigen affinity screening, affinity of antibody to Fc receptors and C1q is responsible for cell killing via ADCC/ADCP and CDC effects respectively. Therefore testing association of Fc receptors and C1q is necessary during the development phase of antibody drug candidates.
Fig4. Standard curve of mouse complement C3a to determine the concentrations of C3a in client mouse plasma samples (SpectraMax i3X).
We can perform a binding assay for Human TROP-2 and a client’s antibody to test the efficacy of the provided antibody using our own Human TROP-2 / TACSTD2 Protein, His Tag (Cat#: TR2-H5223). The microplate is coated overnight with TROP-2. The sample antibody is added to the wells, and subsequently the secondary HRP conjugated Goat Anti-Mouse IgG is added. After the primary antibody from the client binds to TROP-2 the HRP secondary antibody will bind to the primary, and following the addition of TMB the intensity of the signal will be proportional to the concentration of primary antibody.
Fig5. Binding curve comparing the concentration of anti-TROP-2 antibody (nM) to the optical density to determine the EC50 (SpectraMax i3x).
An idiotope is the unique set of antigenic determinants (epitopes) of the variable portion of an antibody. An anti-idiotypic (Anti-ID) antibody binds to the idiotope of another antibody, usually an antibody drug, which makes it a very powerful tool for antibody drug development, especially for immunogenicity and PK/PD analysis.
To support preclinical/clinical immunogenicity and PK analysis, ACROBiosystems has developed a series of high-affinity anti-idiotypic antibodies and ADA/PK services.
Fig6. Immobilized Adali**mab at 1 µg/ml, add increasing concentrations of anti-Adali**mab antibodies (Cat. No. ADB-Y19, 10% human serum) and then add biotinylated Adali**mab at 5 µg/ml. Detection was performed using HRP-conjugated
ACRO has developed conjugated magnetic beads which can be used in beads based ELISA.
ACROBiosystems scientist will respond within 24 hours of submission.
Call us at: +1 800-810-0816 or email at services@acrobiosystems.com for consultation.
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