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| Assay Type | Sandwich-ELISA |
| Analyte | BSA (Bovine serum albumin) |
| Format | 96T |
| Regulatory Status | RUO |
| Sensitivity | ＜0.5ng/mL |
| Standard Curve Range | 0.5ng/mL-40.5ng/mL |
| Assay Time | 1.5 hr |
| Suitable Sample Type | For the quantitative determination of BSA (Bovine serum albumin) |
| Sample volume | 50μL |
product testing require a highly sensitive and reliable analytical method.
The kit is developed for the detection and quantitative determination of BSA. It is used as a tool to aid in optimal purification process development and in routine quality control of in-process streams as well as final product by various biotechnological processes such as cell or tissue culture .
It is for research use only.
2. Find the expiration date on the outside packaging and do not use reagents past their expiration date.
3. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
| ID | Components | Size |
| RES01-C01 | Pre-Coated Anti-BSA Antibody Microplate | 1 plate |
| RES01-C02A | BSA (Bovine serum albumin) Standard (200ng/mL) | 500 μL |
| RES01-C02B | BSA (Bovine serum albumin) Standard 5 (40.5ng/mL) | 500 μL |
| RES01-C02C | BSA (Bovine serum albumin) Standard 4 (13.5ng/mL) | 500 μL |
| RES01-C02D | BSA (Bovine serum albumin) Standard 3 (4.5ng/mL) | 500 μL |
| RES01-C02E | BSA (Bovine serum albumin) Standard 2 (1.5ng/mL) | 500 μL |
| RES01-C02F | BSA (Bovine serum albumin) Standard 1 (0.5ng/mL) | 500 μL |
| RES01-C02G | BSA (Bovine serum albumin) Standard 0 (0ng/mL) | 500 μL |
| RES01-C03 | HRP-Anti-BSA Antibody | 100 μL |
| RES01-C04 | 1×Sample Dilution Buffer | 50 mLX2 |
| RES01-C05 | 20×Washing Buffer | 25 mL |
| RES01-C06 | Substrate Solution | 12 mL |
| RES01-C07 | Stop Solution | 8 mL |
Detection of BSA ( Bovine serum albumin) by ELISA Assay.
Immobilized Anti-BSA Antibody can bind BSA (Bovine serum albumin) Detection was performed using HRP-Anti- BSA Antibody with sensitivity of 0.5 ng/mL (QC tested). For each experiment, a standard curve needs to be set for each micro-plate, and the specific OD value may vary depending on different laboratories, testers, or equipments. The following example data is for reference only.
Three samples of known concentration were tested ten times on one plate to assess intra-assay precision , Intra-Assay Precision CV≤15%.
Three samples of known concentration were tested in ten separate assays to assess inter-assay precision, Inter-Assay Precision CV≤15%.
Five samples of known concentration were tested ten times to assess Accuracy , Accuracy recovery rate 80-120%.
We have conducted interference effect test about frequently-used buffers, they have excellent buffer compatibility. For specific buffers, it is recommended that you verify recovery to determine the minimum dilution ratio.
High, medium and low concentrations of BSA were added to Human serum (v/v 1%), Horse serum (v/v 10%) , Human Serum Albumin (1.25 mg/mL) and Rabbit serum (v/v 10%), the recovery rate of BSA was used as the specific validation index.
High, medium and low concentrations of BSA were added to MDCK( 2×106 cells/mL), HEK293( 3.5×106 cells/mL) , CHO( 2×106 cells/mL) and T-lymphocyte( 2.15×106 cells/mL) the recovery rate of BSA was used as the specific validation index.
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