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Your Position: Casa > Kits > Kanamycin > RES-A004

resDetect™ Kanamycin ELISA Kit

For research use only.

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Product Details
Assay TypeCompetitive-ELISA
Regulatory StatusRUO
Sensitivity0.5 ng/mL
Standard Curve Range0.5 ng/mL-40.5 ng/mL
Assay Time1 hr 20 min
Suitable Sample TypeFor the detection and quantitative determination of kanamycin residues in plasmid DNA raw materials for cell and gene therapy.
Sample volume50 μL
Materials Provided
RES-A004-C01Kanamycin Coated Plate1plate
RES-A004-C02Kanamycin Standard81ng/mL×1.8mL
RES-A004-C03Anti-Kanamycin Antibody6mL
RES-A004-C041×Dilution Buffer50mL
RES-A004-C0520xWashing Buffer 50mL
RES-A004-C06Substrate Solution12mL
RES-A004-C07Stop Solution7mL
  • Background
    Residues of kanamycin are prone to occur in the production process of biological products, which can easily lead to abnormal reactions in the human body. Therefore, the residual amount of kanamycin in biological products or semi-finished products of biological products should be strictly controlled.
  • Application

    The kit is developed for the detection of Kanamycin in drug products or semi-manufactures.

    It is for research use only.

  • Reconstitution
    Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.
  • Storage
    1. The kit should be stored at 2°C to 8°C upon receiving.

    2. The reconstructed kanamycin standard is stored at -70°C in at least 300 uL per tube and cannot be frozen and thawed repeatedly.

    3. Find the expiration date on the outside packaging and do not use reagents past their expiration date.

  • Assay Principles
    The Kanamycin quantitative detection kit adopts the indirect competitive ELISA method, and the pre-coated conjugated kanamycin antigen on the microstrip competes with the residual kanamycin in the sample to bind the enzyme-labeled anti-kanamycin monoclonal antibody, and then uses a microplate reader to detect the absorbance value by adding TMB substrate, and the absorbance value is negatively correlated with the content of kanamycin in the sample. The kit takes only about one hour and 20 minutes to operate and has a linear range of 0.5 ng/mL to 40.5 ng/mL.
Typical Data Please refer to DS document for The assay protocol.

For each experiment, a standard curve needs to be set for each micro-plate, and the specific OD value may vary depending on different laboratories, testers, or equipments. The following example data is for reference only.

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Price(EUR) : €610.00

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