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Proteínas del receptor Fc

Fc receptors-High purity
Introduction

La eficacia de un anticuerpo terapéutico no solo depende del fragmento Fab y su actividad de unión al antígeno objetivo, sino que también depende del fragmento Fc y su interacción con los receptores Fc clave. La afinidad de unión del fragmento Fc hacia FcRn (FCGRT&B2M) predeciría la vida media del anticuerpo, mientras que entre el fragmento Fc y FcγRIIIa (CD16a) influiría en la capacidad del anticuerpo para provocar CMCDA (citotoxicidad mediada por células dependiente de anticuerpos). Por lo tanto, los candidatos deben probarse frente a un panel de receptores durante la ingeniería de anticuerpos.

ACROBiosystems ofrece una colección completa de productos de receptores Fc, incluyendo FcRn. FcγRs y sus variantes comunes, para acelerar el desarrollo de sus anticuerpos monoclonales.

Nueva recomendación de producto: Kit de unión de FcRn humano (TR-FRET)
Es fácil de usar, altamente sensible y preciso en los resultados, lo que lo convierte en una solución ideal para el cribado de alto rendimiento de medicamentos a base de anticuerpos, apoyando y acelerando así el proceso de investigación y desarrollo de anticuerpos monoclonales.

Objetivos requeridos
                                                 
Ventajas de los productos de proteínas del receptor Fc

Expresado por células HEK293: modificación pos-translacional y plegamiento adecuado de la proteína

Múltiples especies: Humanos, ratones, macacos cynomolgus/rhesus, ratas, cerdos, conejos, felinos, bovinos, se pueden aplicar completamente a diferentes experimentos de especies cruzadas

Alta pureza: Pureza de verificación SDS-PAGE>95%, pureza de verificación SEC-MALS>90%

Baja endotoxina:<1,0 UE/µg                    

Alta estabilidad: estricto control de calidad para garantizar una alta consistencia entre lotes

Se ofrecen proteínas del receptor Fc biotiniladas etiquetadas con AvitagTM la eficiencia de etiquetado es alta, y el sitio de etiquetado es específico y claro, lo que es adecuado para la detección ELISA/SPR/BLI basada en la unión a la estreptavidina en el proceso de desarrollo y optimización de medicamentos

Afinidad verificada por SPR y BLI: actividad garantizada, y protocolos ofrecidos gratuitamente

Características claves

High purity

To meet the high purity requirement of pharmaceutical applications, our production team perform both SDS-PAGE and SEC-MALS analyses to the Fc receptors for QC purposes. It’s noteworthy that it’s essential to use monomeric Fc receptor, which represents its natural state. The formation of oligomers during production may result in artificially enhanced binding interaction due to avidity effect. To avoid such situation, we have established strict internal control standards of monomer purity by SEC-MALS testing. Only those batches meeting all purity requirements are released.

Fc receptors-SPR

Fig. 1 The purity of Human CD32a (R167), His Tag (Cat. No. CDA-H5221) is more than 95% verified by SDS-PAGE. The purity of Human CD32a (R167), His Tag (Cat. No. CDA-H5221) is more than 90% verified by SEC-MALS. And the molecular weight of this protein is around 25-35 kDa.

Bioactividad validada por SPR&BLI

The binding affinity between two molecules can be determined by many methods. However, the studies of Fc interaction are often done by SPR, because traditional ELISA is not appropriate for low affinity receptors such as CD16 and CD32. To assure our Fc receptors deliver expected performance, our QC team use Biacore platform to test our products against reference antibodies. Additionally, to meet more applications in the development of antibody drugs, our QC team also use ForteBio platform to test our products against reference antibodies. All SPR&BLI protocols are available for free.

Fc receptors-SPR

Fig. 2 Human CD64, His Tag (SPR & BLI verified) (Cat. No. FCA-H52H1) captured on CM5 chip via anti-His antibody can bind Herc*ptin with an affinity constant of 4.92 nM as determined in a SPR assay (Biacore 8K) (QC tested).

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Fc receptors-BLI

Fig. 3 Immobilized Human FCGRT&B2M Heterodimer Protein, His Tag&Strep II Tag (SPR & BLI verified) (Cat. No.  FCM-H5286) on CM5 Chip via Anti-His antibody, can bind Herc*ptin with an affinity constant of 0.261 μM as determined in a SPR assay (Biacore T200).

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Fc receptors-BLI

Fig. 4 Loaded Human CD64, His Tag (SPR & BLI verified) (Cat. No. FCA-H52H1) on HIS1K Biosensor, can bind Herc*ptin with an affinity constant of 6.98 nM as determined in BLI assay (ForteBio Octet Red96e).

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Fc receptors-BLI

Fig. 5 Loaded Human FCGRT&B2M Heterodimer Protein, His Tag (SPR & BLI verified) (Cat. No. FCN-H52W7) on SA Biosensor via Biotin his antibody, can bind Herc*ptin with an affinity constant of 0.14 μM as determined in BLI assay (ForteBio Octet Red96e).

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Alta consistencia de lote a lote

We routinely apply rigorous quality control measures to ensure consistent performance of our product. As shown below, the batch variation among the tested samples is negligible.

Fc receptors-High batch to batch consistency

Fig. 6 Immobilized Human Fc gamma RIIB / CD32b Protein (Cat. No. CDB-H5228) on CM5 Chip via anti-His antibody, can bind Ritux*mab with an affinity constant of 10 μM as determined in a SPR assay (Biacore T200).

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We have compared the performance of different batches of CD32b (CDB-H5228) in the same assay, and the software analysis showed that the similarity score was very high, meaning high batch-to-batch consistency.

Fc receptors-High batch to batch consistency

Fig. 7 Batch consistency of Human Fc gamma RIIB / CD32b (Cat. No. CDB-H5228). The consistency of different batches is more than 90%.

Receptores Fc biotinilados o de otras especies disponibles

The use of biotin labeling can make your assay development much easier. We offer a variety of ready-to-use biotinylated Fc receptors. These proteins are produced using our in-house developed labeling techniques, which confers high bioactivity and minimal batch-to-batch variation. In addition, we have developed a series of Fc receptors of other species, which are suitable for the screening of non-humanized antibodies or the species cross reaction.

Fc receptors-High batch to batch consistency

Fig. 8 Biotinylated Human CD16a (V176), His Tag, Avi Tag (Cat. No.CDA-H82E9) captured on Biotin CAP- Series S Sensor Chip can bind Ritux*mab with an affinity constant of 0.261 μM as determined in a SPR assay (Biacore T200).

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Fc receptors-High batch to batch consistency

Fig. 9 Immobilized Cynomolgus / Rhesus macaque FcRn Protein (Cat. No. FCM-C5284) on CM5 Chip via anti-His antibody, can bind Herc*ptin with an affinity constant of 0.403 μM as determined in a SPR assay (Biacore T200).

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Lista de productos
(Todos los receptores Fc se expresan a partir de HEK293)
  • FcRn

  • FcγR

  • FcεR

MoléculaCat. No.AnfitriónDescripción del productoEstructura
MoleculeCat. No.HostProduct DescriptionStructure
MoleculeCat. No.HostProduct DescriptionStructure

Moléculas relacionadas con el receptor Fc-proteína IgG Fc

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