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Your Position: Casa > Antibody > gH&gL > GHL-MY2111

Monoclonal Anti-VZV gH & gL Antibody, Human IgG1 (4F12) (MALS verified)

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  • Source

    Monoclonal Anti-VZV gH & gL Antibody, Human IgG1 (4F12) is a chimeric monoclonal antibody recombinantly expressed from HEK293, which combines the variable region of a mouse monoclonal antibody with Human constant domain.

  • Clone

    4F12

  • Species

    Mouse

  • Isotype

    Human IgG1 | Human Kappa

  • Conjugate

    Unconjugated

  • Antibody Type

    Recombinant Monoclonal

  • Reactivity

    Virus

  • Immunogen

    Recombinant Varicella zoster virus (strain Oka vaccine) gH&gL Protein is expressed from human 293 cells.

  • Specificity

    Specifically recognizes VZV gH & gL.

  • Application
    ApplicationRecommended Usage
    ELISA0.1-63 ng/mL
  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Purification

    Protein A purified/ Protein G purified

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
gH&gL SDS-PAGE

Monoclonal Anti-VZV gH & gL Antibody, Human IgG1 (4F12) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

SEC-MALS
gH&gL MALS images

The purity of Monoclonal Anti-VZV gH & gL Antibody, Human IgG1 (4F12) (Cat. No. GHL-MY2111) is more than 90% and the molecular weight of this protein is around 135-165 kDa verified by SEC-MALS.

Bioactivity-ELISA
 gH&gL ELISA

Immobilized Varicella zoster virus (strain Oka vaccine) gH&gL Protein, Twin-Strep Tag&His Tag (Cat. No. GHL-V5283) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-VZV gH & gL Antibody, Human IgG1 (4F12) (Cat. No. GHL-MY2111) with a linear range of 0.1-4 ng/mL (QC tested).

  • Background
    Varicella-zoster virus (VZV) is the alphaherpesvirus that causes chicken pox (varicella) and shingles (zoster). As for all herpesviruses, VZV relies on a fusion complex comprised of three core glycoproteins, gB, gH and gL, required for entry of virions into host cells. VZV ORFs 37 and 60 encode gH (841aa; 118 kDa) and gL (160aa; 20 kDa), respectively, which are N- and O-linked glycosylated and form a heterodimer that is necessary for the activation of gB.
  • Clinical and Translational Updates

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Price(EUR) : €350.00

Price(EUR) : €2330.00

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