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| ID | Components | Size |
| EP168-C01 | High-bind Plate | 1 plate |
| EP168-C02 | Human DR3 | 100 μg |
| EP168-C03 | Biotinylated Human TL1A | 6 μg |
| EP168-C04 | Human Anti-TL1A Neutralizing Antibody | 100 μg |
| EP168-C05 | Streptavidin-HRP | 5 μg |
| EP168-C06 | Coating Buffer | 12mL |
| EP168-C07 | 20xWashing Buffer | 50mL |
| EP168-C08 | Blocking Buffer | 50mL |
| EP168-C09 | Substrate Solution | 12mL |
| EP168-C10 | Stop Solution | 7mL |
This kit is developed for screening for inhibitors of human TL1A binding to human DR3.
It is for research use only.
The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
Your experiment will include 4 simple steps:
1) Coat the plate with Human DR3.
2) Add your molecule of interest to the tests.
3) Add Human TL1A-Biotin to bind the coated Human DR3.
4) Add Streptavidin-HRP followed by TMB or other colorimetric HRP substrate.
Finally, the half maximal inhibitory concentration (IC50) of your compound to Human TL1A: Human DR3 binding will be determined by comparing OD readings among different experimental groups.
Serial dilutions of Human Anti-TL1A Neutralizing antibody (Catalog # EP168-C04) (1:1 serial dilution, from 20μg/mL to 0.3125μg/mL) was added into Human DR3: Biotinylated Human TL1A binding reactions. The assay was performed according to the protocol described below. Background was subtracted from data points prior to log transformation and curve fitting (QC tested).
For each experiment, a standard curve needs to be set for each micro-plate, and the specific OD value may vary depending on different laboratories, testers, or equipments. The example data is for reference only.
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