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Your Position: Casa > Cell > FcRn > SCMDC-ATP196

MDCK/Mouse FCGRT-P2A-mGFP&B2M Cell Line Development Service

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  1. Genetically modified cell lines best reflect MOA (Mechanism of Action)
  2. Higher activity and larger assay window for robust and reproducible cell-based bioassay
  3. Comprehensive application data to support assay development and validation
  4. Full tracible record, stringent quality control and validated cell passage stability
  5. Parental cell line legally obtained from internationally recognized cell resource bank and commercially licensed
  6. Global commercial license assistance whenever regulatory filing is required
  • Description
    The MDCK/Mouse FCGRT-P2A-mGFP&B2M Cell Line was engineered to not only express full length mouse FCGRT (Gene ID: 14132) and mGFP by a viral P2A self-cleaving peptide, but also express full length mouse B2M (Gene ID: 12010). The expression of mGFP characterizing the mouse FCGRT was confirmed by flow cytometry. Surface expression of mouse B2M was confirmed by flow cytometry.
  • Application

    •        Useful for cell-based mouse FCGRT-P2A-mGFP&B2M binding assay

  • Growth Properties
    Adherent
  • Selection Marker
    Puromycin (2 μg/mL) + Hygromycin (20 μg/mL)
  • Culture Medium
    EMEM + 10% FBS
  • Freeze Medium
    Serum-free cell cryopreservation medium
  • Quantity
    1 vial contains at least 5×10^6 cells in 1 mL serum-free cryopreservation medium
  • Storage
    Frozen in liquid nitrogen.
  • Mycoplasma Testing
    Negative
  • Sterility Testing
    Negative
  • Instructions for Use
    See data sheet for detailed culturing and assay protocol.
Receptor Assay
 FcRn FACS

Fig1. Expression analysis of mouse FCGRT on MDCK/Mouse FCGRT-P2A-mGFP&B2M Stable Cell Line by FACS.
The expression of mGFP characterizing the mouse FCGRT was detected by flow cytometry.

 FcRn FACS

Fig2. Expression analysis of mouse B2M on MDCK/Mouse FCGRT-P2A-mGFP&B2M Stable Cell Line by FACS.
Cell surface staining was performed on MDCK/Mouse FCGRT-P2A-mGFP&B2M Stable Cell Line or negative control cell using APC-labeled anti-mouse β2-microglobulin antibody.

Please contact us if you are interested in related cell pool service.

  • Background
    AC2294C3
  • Limited Use&License Disclosure
    1. This cell line is provided for research use only. It is neither intended for any animal or human therapeutic use nor for any direct human in vivo use. You are restricted to share, modify, transfer, distribute, sell, sublicense, or otherwise make the cell line available for use to other researchers, laboratories, research institutions, hospitals, universities, or service organizations.
    2. ACROBIOSYSTEMS MAKES NO WARRANTIES OR REPRESENTATIONS OF ANY KIND, EITHER EXPRESSED OR IMPLIED, WITH RESPECT TO THE SUITABILITY OF THE CELL LINE FOR ANY PARTICULAR USE.
    3. ACROBIOSYSTEMS ACCEPTS NO LIABILITY IN CONNECTION WITH THE HANDLING OR USE OF THE CELL LINE.
    4. Modifications of the cell line, transfer to a third party, or commercial use of the cell line may require a separate license and additional fees. Please contact order@acrobiosystems.com for further details.
  • Clinical and Translational Updates

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ETA de productos en stock: 2 días laborables

Price(EUR) : €3540.00

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Drug Development Status

  • Number of Launched Drugs:0 Details
  • Number of Drugs in Clinical Trials:6 Details
  • Latest Research Phase:Phase ?

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