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| Assay Type | Sandwich-ELISA |
| Analyte | Laminin |
| Format | 96T |
| Reactivity | Human |
| Regulatory Status | RUO |
| Sensitivity | ＜3.125 ng/mL |
| Standard Curve Range | 3.125 ng/mL-100 ng/mL |
| Assay Time | 3 hr 20 min |
| Suitable Sample Type | For the quantitative determination of Laminin in human serum and cell culture supernates. |
| Sample volume | 100 uL |
| ID | Components | Size |
| RES091-C01 | Pre-coated Anti-Laminin Antibody Microplate | 1 plate |
| RES091-C02 | Human Laminin 521 Standard | 20 μg |
| RES091-C03 | Biotin-Anti-Laminin Antibody | 20 μg |
| RES091-C04 | Streptavidin-HRP | 50 μL |
| RES091-C05 | 10×Washing Buffer | 50 mL |
| RES091-C06 | 2×Dilution Buffer | 50 mL |
| RES091-C07 | Substrate Solution | 12 mL |
| RES091-C08 | Stop Solution | 7 mL |
To support the development of cell therapy drugs, ACROBiosystems developed Human Laminin quantitive ELISA Kit with rigorous methodological validation, which is used for the detection and quantitative determination of Laminin in cell culture supernates, serum, and plasma. Besides, this kit can also be used for the quantitative determination of GMP human Laminin 521 Protein (ACROBiosystems, cat#GMP-LA5H24) concentrations.
The kit is developed for the detection and quantitative determination of Laminin in cell culture supernates, serum, and plasma.
This kit can specifically identify full length Laminin521, Laminin521 E8 fragment, full length Laminin511 and Laminin511 E8 fragment by testing.
It is for research use only.
2. Find the expiration date on the outside packaging and do not use reagents past their expiration date.
3. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
Your experiment will include 6 simple steps:
a) Bring all reagents to room temperature(20℃-25℃) before use.
b) Add your sample to the plate and take the Human Laminin 521 as standard. The samples and standard are diluted by Dilution Buffer.
c) Wash the plate and add the Biotin-Anti-Laminin Antibody diluted by Dilution Buffer to the plate.
d) Wash the plate and add the Streptavidin-HRP diluted by Dilution Buffer to the plate.
e) Wash the plate and add TMB.
f) Stop the substrate reaction by adding diluted acid. Absorbance (OD) is calculated by the absorbance at 450 nm minus the absorbance at 630 nm to remove background disturbance before statistical analysis. The OD Value reflects the amount of bound protein.
For each experiment, a standard curve needs to be set for each micro-plate, and the specific OD value may vary depending on different laboratories, testers, or equipments. The following example data is for reference only.
To assess the linearity of the assay, samples spiked with high concentrations of Laminin 521 were serially diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.
Three samples of known concentration were tested ten times on one plate to assess intra-assay precision.
Three samples of known concentration were tested in three separate assays to assess inter-assay precision.
Three Laminin 521 with different concentrations were tested to calculate the recovery rate.
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