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The kit is developed for quantitative detection of natural and recombinant human PCSK9 in serum, plasma and cell culture supernatants.
It is for research use only.
| Analyte | PCSK9 |
| Assay Type | Sandwich-ELISA |
| Reactivity | Human |
| Sensitivity | 0.45 ng/ml |
| Range | 0.625 ng/mL-20 ng/mL |
| Assay Time | 2.75 hr |
| Sample Type | Cell Culture Supernatants, Plasma, Serum. |
| Sample volume | 50 uL |
| Format | 96-wells plate breakable into 12 x 8 wells strips |
Elevate your research experience with our Cytokine/Biomarker Detection Kits, where accuracy, reliability, and ease of use are converging to deliver exceptional results.
For each experiment, a standard curve needs to be set for each microplate, and the specific OD value may vary depending on different laboratories, testers, or equipment. The following example data is for reference only. The sample concentration was calculated based on the results of the standard curve. The minimum detectable concentration of PCSK9 is less than 0.45 ng/mL.
Serum – PCSK9 concentrations were measured in 24 human serum samples (dilution ratio 1:40), sample data shows in the figure below.
High concentrations of human PCSK9 serum samples were diluted with 1:2, 1:4, 1:8 and 1:16 ratios for gradient dilution to evaluate the linearity of the assay. In the serum samples, the average detection rate of PCSK9 was 100.6%.
Ten replicates of each of 3 samples containing different PCSK9 concentrations were tested in one assay. Acceptable criteria: CV＜10%.
3 samples containing different concentrations of PCSK9 were tested in independent assays. Acceptable criteria: CV<15%.
Recombinant PCSK9 was spiked into 3 human serum samples, and then analyzed. The average recovery of PCSK9 for serum samples is 96.4%.
| ID | Components | Size |
| CEA202-C01 | Pre-coated Anti-PCSK9 Antibody Microplate | 1 plate |
| CEA202-C02 | Human PCSK9 Standard | 20 μg×2 |
| CEA202-C03 | Biotin-Anti-PCSK9 Antibody Con. Solution | 100 μL |
| CEA202-C04 | Biotin-Antibody Dilution Buffer | 8 mL |
| CEA202-C05 | Streptavidin-HRP Con. Solution | 500 μL |
| CEA202-C06 | Streptavidin-HRP Dilution Buffer | 15 mL |
| CEA202-C07 | 20× Washing Buffer | 50 mL |
| CEA202-C08 | Sample Dilution Buffer | 15 mL×2 |
| CEA202-C09 | Substrate Solution | 12 mL |
| CEA202-C10 | Stop Solution | 6 mL |
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