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Limited Edition Golden Llama is here! Check out how you can get one. 
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The kit is developed for quantitative detection of natural and recombinant human VEGF/VEGF-A in serum, plasma and cell culture supernatants.
It is for research use only.
| Analyte | VEGF |
| Assay Type | Sandwich-ELISA |
| Reactivity | Human |
| Sensitivity | <7pg/mL |
| Range | 31.25 pg/mL-2000 pg/mL |
| Assay Time | 3 hr 10 min |
| Sample Type | Cell Culture Supernatants, Plasma, Serum. |
| Sample volume | 100 uL |
| Format | 96-wells plate breakable into 12 x 8 wells strips |
Elevate your research experience with our Cytokine/Biomarker Detection Kits, where accuracy, reliability, and ease of use are converging to deliver exceptional results.
For each experiment, a standard curve needs to be set for each microplate, and the specific OD value may vary depending on different laboratories, testers, or equipment. The following example data is for reference only. The sample concentration was calculated based on the results of the standard curve. The minimum detectable concentration of VEGF is less than 7 pg/mL.
Ten replicates of each of 3 samples containing different VEGF concentrations were tested in one assay. Acceptable criteria: CV＜10%.
3 samples containing different concentrations of VEGF were tested in independent assays. Acceptable criteria: CV<15%.
Recombinant VEGF was spiked into 3 human serum samples, and then analyzed. The average recovery of VEGF for serum samples is 85.87%.
| ID | Components | Size |
| CEA068-C01 | Pre-coated Anti-VEGF Antibody Microplate | 1 plate |
| CEA068-C02 | Human VEGF Standard | 2000 pg×2 |
| CEA068-C03 | Biotin-Anti-VEGF Antibody Con. Solution | 120 μL |
| CEA068-C04 | Biotin-Antibody Dilution Buffer | 15 mL |
| CEA068-C05 | Streptavidin-HRP Con. Solution | 120 μL |
| CEA068-C06 | Streptavidin-HRP Dilution Buffer | 15 mL |
| CEA068-C07 | 20× Washing Buffer | 25 mL |
| CEA068-C08 | Sample Dilution Buffer | 15 mL×2 |
| CEA068-C09 | Substrate Solution | 15 mL |
| CEA068-C10 | Stop Solution | 15 mL |
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