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Your Position: Casa > Protein > HLA-DRA1*0101 & HLA-DRB1*0401 > HL1-H82E5

Biotinylated Human HLA-DRA1*01:01&HLA-DRB1*04:01 Monomer Protein (Peptide free, MALS verified)

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  • Source
    Biotinylated Human HLA-DRA1*01:01&HLA-DRB1*04:01 Monomer Protein(HL1-H82E5) is expressed from human 293 cells (HEK293). It contains AA Ile 26 - Glu 216 (HLA-DRA1*01:01) & Gly 30 - Lys 227 (HLA-DRB1*04:01) (Accession # CAI2388006.1 (HLA-DRA1*01:01) & CAI9239961.1 (HLA-DRB1*04:01)).
    Predicted N-terminus: Ile 26 & Gly 30
  • Molecular Characterization

    This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 29.2 kDa and 28.8 kDa. The protein migrates as 35-40 kDa and 30-33 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Labeling
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Protein Ratio
    Passed as determined by the HABA assay / binding ELISA.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
HLA-DRA1*0101 & HLA-DRB1*0401 SDS-PAGE

Biotinylated Human HLA-DRA1*01:01&HLA-DRB1*04:01 Monomer Protein on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

SEC-MALS
HLA-DRA1*0101 & HLA-DRB1*0401 MALS images

The purity of Biotinylated Human HLA-DRA1*01:01&HLA-DRB1*04:01 Monomer Protein (Cat. No. HL1-H82E5) is more than 90% and the molecular weight of this protein is around 58-73 kDa verified by SEC-MALS.

Bioactivity-ELISA
 HLA-DRA1*0101 & HLA-DRB1*0401 ELISA

Immobilized Biotinylated Human HLA-DRA1*01:01&HLA-DRB1*04:01 Monomer Protein (Cat. No. HL1-H82E5) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Anti-HLA-DR Antibody, Human IgG1 with a linear range of 0.06-2 ng/mL (QC tested).

  • Clinical and Translational Updates

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ETA de productos en stock: 2 días laborables

Price(EUR) : €120.00

Price(EUR) : €690.00

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