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Your Position: Casa > Protein > PD-L2 > PD2-M82E3

Biotinylated Mouse PD-L2 / B7-DC Protein, His,Avitag™ (MALS verified)

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  • Synonym
    PDL2,PD-L2,Butyrophilin B7-DC,CD273,PDCD1 ligand 2,PDCD1L2,PDCD1LG2
  • Source
    Biotinylated Mouse PD-L2 Protein, His,Avitag(PD2-M82E3) is expressed from human 293 cells (HEK293). It contains AA Leu 20 - Arg 219 (Accession # Q9WUL5).
    Predicted N-terminus: Leu 20
  • Molecular Characterization
    PD-L2 Structure

    This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 26.1 kDa. The protein migrates as 35-43 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Labeling
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Protein Ratio
    Passed as determined by the HABA assay / binding ELISA.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
PD-L2 SDS-PAGE

Biotinylated Mouse PD-L2 Protein, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

SEC-MALS
PD-L2 MALS images

The purity of Biotinylated Mouse PD-L2 Protein, His,Avitag (Cat. No. PD2-M82E3) is more than 90% and the molecular weight of this protein is around 30-40 kDa verified by SEC-MALS.

Bioactivity-ELISA
 PD-L2 ELISA

Immobilized Biotinylated Mouse PD-L2 Protein, His,Avitag (Cat. No. PD2-M82E3) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Mouse PD-1 Protein, Mouse IgG2a Fc Tag (Cat. No. PD1-M5257) with a linear range of 1.2-10 ng/mL (QC tested).

 PD-L2 ELISA

Immobilized Human PD-1, Mouse IgG2a Fc Tag (Cat. No. PD1-H5255) at 5 μg/mL (100 μL/well) can bind Biotinylated Mouse PD-L2 Protein, His,Avitag (Cat. No. PD2-M82E3) with a linear range of 2-78 ng/mL (Routinely tested).

  • Background
    Programmed cell death 1 ligand 2 (PD-L2 or PDCD1 ligand 2) is also known as Butyrophilin B7-DC, CD antigen CD273, which belongs to the immunoglobulin superfamily or BTN/MOG family. The expression of PD-L2 is up-regulated by IFNG/IFN-gamma stimulation in monocytes and induced on dendritic cells grown from peripheral blood mononuclear cells with CSF2 and IL-4. PD-L2 Involved in the costimulatory signal, essential for T-cell proliferation and IFNG production in a PDCD1-independent manner. PD-L2 interaction with PDCD1 inhibits T-cell proliferation by blocking cell cycle progression and cytokine production.
  • Clinical and Translational Updates

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