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Assay Type | Inhibition-TR-FRET |
Analyte | FcRn binding component |
Format | 100T, 500T, 10000T |
Reactivity | Human |
Regulatory Status | RUO |
Sensitivity | IC50=20.14μg/mL |
Standard Curve Range | 0.24 μg/mL-1000 μg/mL |
Assay Time | 1 hr |
Suitable Sample Type | For the binding of IgG Fc region to the human FcRn receptor |
Sample volume | 10 μL |
The Human FcRn Binding Kit (TR-FRET) can detect the binding of IgG Fc region to the human FcRn receptor in homogeneous system within 0.5-1 hours. it is high sensitive, short detection time and easy to use.
The kit is developed for the binding of IgG Fc region to the human FcRn receptor. It is used as a universal detection tool to identifying the ability of antibody drugs to bind to FcRn.
It is for research use only.
2. Find the expiration date on the outside packaging and do not use reagents past their expiration date.
3. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
ID | Components | Size |
FRT01-C01 | Human FCRN&B2M Heterodimer Protein Europium-chelate | 2.7 μg/100 tests 13.5 μg/500 tests 270 μg/10000 tests |
FRT01-C02 | FA labeled human IgG antibody | 2.4 μg/100 tests 12 μg/500 tests 240 μg/10000 tests |
FRT01-C03 | Human IgG standard | 200 μg/100 tests 1 mg/500 tests 20 mg/10000tests |
FRT01-C04 | Sample Dilution Buffer | 10 mL/100tests & 500tests 100 mL/10000tests |
FRT01-C05 | Detection Buffer | 10 mL/100tests & 500tests 100 mL/10000tests |
Inhibition of Europium-chelate labeled FcRn: FA labeled human IgG antibody binding by Human IgG
Premix serial dilutions of Human IgG standard (Catalog # FRT01-C03) (1:4 serial dilution, from 1000 μg/mL to 0.24 μg/mL (6666.67-1.63 nM)) and Human FCRN&B2M Heterodimer Protein Europium-chelate (Catalog # FRT01-C01) and incubate at room temperature (20℃-25℃) for 0.5 hour. Then add FA labeled human IgG antibody (Catalog # FRT01-C02) and incubate at room temperature (20℃-25℃) for 0.5 hour. Detection was performed with IC50 of 20.14 μg/mL. The assay was performed according to the above-described Datasheet (QC tested).
The kit has been used to detect different human IgG isoforms of Human IgG1, Human IgG2, Human IgG3, Human IgG4, and modified Human IgG1 Fc (C103S, M135Y, S137T, T139E, H316K, N317F) Protein, His Tag (Cat. No. IG1-H52H8) with different affinities binding to FcRn.
The kit has been used to detect the binding activity between Human FcRn and Human IgG standard (FRT-C03) under different pH. The affinity between Human FcRn and Human IgG standard (FRT-C03) decreases with increasing pH.
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