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The kit is developed for quantitative detection of natural and recombinant human IL-12/IL-23 p40 in serum, plasma and cell culture supernatants.
It is for research use only.
| Analyte | IL-12/IL-23 p40 |
| Assay Type | Sandwich-ELISA |
| Reactivity | Human |
| Sensitivity | <15.625 pg/mL |
| Range | 31.25 pg/mL-2000 pg/ml |
| Assay Time | 2hr |
| Sample Type | Cell Culture Supernatants, Plasma, Serum. |
| Sample volume | 50 μL |
| Format | 96-wells plate breakable into 12 x 8 wells strips |
Elevate your research experience with our Cytokine/Biomarker Detection Kits, where accuracy, reliability, and ease of use are converging to deliver exceptional results.
For each experiment, a standard curve needs to be set for each microplate, and the specific OD value may vary depending on different laboratories, testers, or equipment. The following example data is for reference only. The sample concentration was calculated based on the results of the standard curve. The minimum detectable concentration of IL-12/IL-23 p40 is less than 15.625 pg/mL.
Ten replicates of each of 4 samples containing different IL-12/IL-23 p40 concentrations were tested in one assay. Acceptable criteria: CV＜10%.
Five samples containing different concentrations of IL-12/IL-23 p40 were tested in independent assays. Acceptable criteria: CV<15%.
Recombinant IL-12/IL-23 p40 was spiked into 3 human serum samples, and then analyzed. The average recovery of IL-12/IL-23 p40 for serum samples is 103.2%.
| ID | Components | Size |
| CEA203-C01 | Pre-coated Anti- IL-12/IL-23 p40 Antibody Microplate | 1 plate |
| CEA203-C02 | Human IL-12/IL-23 p40 Standard | 20μg ×2 |
| CEA203-C03 | Biotin-Anti-IL-12/IL-23 p40 Antibody Con. Solution | 100 μL |
| CEA203-C04 | Biotin-Antibody Dilution Buffer | 8 mL |
| CEA203-C05 | Streptavidin-HRP Con. Solution | 500 uL |
| CEA203-C06 | Streptavidin-HRP Dilution Buffer | 15 mL |
| CEA203-C07 | 20× Washing Buffer | 50 mL |
| CEA203-C08 | Sample Dilution Buffer | 15 mL ×2 |
| CEA203-C09 | Substrate Solution | 12 mL |
| CEA203-C10 | Stop Solution | 6 mL |
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