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The kit is developed for quantitative detection of natural and recombinant human NT5E/CD73 in serum, plasma and cell culture supernatants.
It is for research use only.
| Analyte | Soluble NT5E/CD73 |
| Assay Type | Sandwich-ELISA |
| Reactivity | Human |
| Sensitivity | 100 pg/mL |
| Range | 123 pg/mL-30000 pg/mL |
| Assay Time | 3hr |
| Sample Type | Cell Culture Supernatants, Plasma, Serum. |
| Sample volume | 100 μL |
| Format | 96-wells plate breakable into 12 x 8 wells strips |
Elevate your research experience with our Cytokine/Biomarker Detection Kits, where accuracy, reliability, and ease of use are converging to deliver exceptional results.
For each experiment, a standard curve needs to be set for each microplate, and the specific OD value may vary depending on different laboratories, testers, or equipment. The following example data is for reference only. The sample concentration was calculated based on the results of the standard curve. The minimum detectable concentration of NT5E/CD73 is less than 100 pg/mL.
Ten replicates of each of 3 samples containing different NT5E/CD73 concentrations were tested in one assay. Acceptable criteria: CV＜10%.
3 samples containing different concentrations of NT5E/CD73 were tested in independent assays. Acceptable criteria: CV<15%.
Recombinant NT5E/CD73 was spiked into 3 human serum samples, and then analyzed. The average recovery of NT5E/CD73 for serum samples is 93.0%.
| ID | Components | Size |
| CEA048-C01 | Pre-coated Anti- Soluble NT5E/CD73 Antibody Microplate | 1 plate |
| CEA048-C02 | Human Soluble NT5E/CD73 Standard | 123μg ×2 |
| CEA048-C03 | Biotin-Anti-Soluble NT5E/CD73 Antibody Con. Solution | 200 μL |
| CEA048-C04 | Biotin-Antibody Dilution Buffer | 15 mL |
| CEA048-C05 | Streptavidin-HRP Con. Solution | 500 uL |
| CEA048-C06 | Streptavidin-HRP Dilution Buffer | 15 mL |
| CEA048-C07 | 20× Washing Buffer | 50 mL |
| CEA048-C08 | Sample Dilution Buffer | 15 mL ×2 |
| CEA048-C09 | Substrate Solution | 12 mL |
| CEA048-C10 | Stop Solution | 6 mL |
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