0
No hay productos en el carro de la compra
A B C D E F G H I J K L M N O P Q R S T U V W X Y Z 0-9
Your Position: Casa > Protein > HLA-A*0301 & B2M & gp100 (ALLAVGATK) > HLG-H82E3

Biotinylated Human HLA-A*03:01&B2M&gp100 (ALLAVGATK) Complex Protein (Monomer, MALS verified)

Streptavidin Protein - PE (STN-NP117, STN-NP119), supporting the assembly of your own MHC Tetramers!

Order Now

  • Source
    Biotinylated Human HLA-A*03:01&B2M&gp100 (ALLAVGATK) Complex Protein(HLG-H82E3) is expressed from human 293 cells (HEK293). It contains AA Gly 25 - Thr 305 (HLA-A*03:01) & Ile 21 - Met 119 (B2M) & ALLAVGATK peptide (Accession # P04439 (HLA-A*03:01) & P61769 (B2M) & ALLAVGATK).
    Predicted N-terminus: Gly 25 & Ile 21
  • Molecular Characterization

    Biotinylated Human HLA-A*03:01&B2M&gp100 (ALLAVGATK) Complex Protein is produced by co-expression of HLA and B2M loaded with gp100 peptide.

    This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 35.9 kDa and 11.7 kDa. The protein migrates as 40-43 kDa and 10 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Labeling
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Protein Ratio
    Passed as determined by the HABA assay / binding ELISA.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
HLA-A*0301 & B2M & gp100 (ALLAVGATK) SDS-PAGE

Biotinylated Human HLA-A*03:01&B2M&gp100 (ALLAVGATK) Complex Protein on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

SEC-MALS
HLA-A*0301 & B2M & gp100 (ALLAVGATK) MALS images

The purity of Biotinylated Human HLA-A*03:01&B2M&gp100 (ALLAVGATK) Complex Protein (Cat. No. HLG-H82E3) is more than 90% and the molecular weight of this protein is around 40-60 kDa verified by SEC-MALS.

Bioactivity-ELISA
 HLA-A*0301 & B2M & gp100 (ALLAVGATK) ELISA

Immobilized Biotinylated Human HLA-A*03:01&B2M&gp100 (ALLAVGATK) Complex Protein (Cat. No. HLG-H82E3) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Anti-HLA class I Antibody, Human IgG1 (W6/32) with a linear range of 0.1-1 ng/mL (QC tested).

 HLA-A*0301 & B2M & gp100 (ALLAVGATK) ELISA

Immobilized Biotinylated Human HLA-A*03:01&B2M&gp100 (ALLAVGATK) Complex Protein (Cat. No. HLG-H82E3) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Anti-B2M Antibody, Human IgG1 with a linear range of 0.1-1 ng/mL (Routinely tested).

  • Background
    Glycoprotein 100, is also well known as gp100 and Melanocyte protein PMEL. This antigen is a melanocyte-specific type I transmembrane glycoprotein and composed of 661 amino acids. Glycoprotein 100 is related to the tumor regression in metastatic melanoma patients after adoptive therapy, by which its epitopes being recognized by tumor lymphocytes and T-cells. The gp100280–288(YLEPGPVTA) was originally shown to be recognized by HLA-A*0301 tumor-infiltrating lymphocytes from melanoma patients, and therefore it is widely been studied in TCR -T studies. The Human HLA-A*0301 gp100 (YLEPGPVTA) complex protein is a complex of HLA-A*0301 of the MHC Class I, B2M and YLEPGPVTA peptide of the gp100.
  • Clinical and Translational Updates

Comments (0)


ETA de productos en stock: 2 días laborables

Price(EUR) : €120.00

Price(EUR) : €690.00

Recommended Products
Placa recubierta de estreptavidina

Price(EUR) : €30.00

Price(EUR) : €140.00

Estreptavidina-HRP

Price(EUR) : €50.00

Price(EUR) : €120.00

Perlas magnéticas™ Estreptavidina

Price(EUR) : €140.00

Price(EUR) : €230.00

Promotion & Exhibitions



Datasheet & Documentation

Request for DMF
DMF (Drug Master File)

New Product Launch

Questions & Comments

This web search service is supported by Google Inc.

totop
Email
Correo electrónico
Fax
Fax
+1 888-377-6111
Address
Address
Lichtstrasse 35,4056 Basel, Switzerland

Dejar un mensaje