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Your Position: Casa > Protein > FUS > FUS-H5144

Human FUS Protein, His Tag

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  • Synonym
    75 kDa DNA-pairing protein,ALS6,amyotrophic lateral sclerosis 6,FUS1,fused in sarcoma,fus-like protein,Oncogene FUS,Oncogene TLS,RNA-binding protein FUS,POMP75,Translocated in liposarcoma,Translocated in liposarcoma protein
  • Source
    Human FUS Protein, His Tag(FUS-H5144) is expressed from E. coli cells. It contains AA Met 1 - Tyr 526  (Accession # P35637-1).
    Predicted N-terminus: Met
  • Molecular Characterization
    FUS Structure

    This protein carries a polyhistidine tag at the N-terminus.

    The protein has a calculated MW of 97.0 kDa. The protein migrates as 95 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE).

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >85% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
FUS SDS-PAGE

Human FUS Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 85% (With Star Ribbon Pre-stained Protein Marker).

Bioactivity-ELISA
 FUS ELISA

Immobilized Human FUS Protein, His Tag (Cat. No. FUS-H5144) at 1 μg/mL (100 μL/well) can bind FUS antibody, Mouse IgG with a linear range of 0.2-4 ng/mL (QC tested).

  • Background
    FUS (R-binding protein FUS) is a D/R-binding protein that plays a role in various cellular processes such as transcription regulation, R splicing, R transport, D repair and damage response. It binds to nascent pre-mRs and acts as a molecular mediator between R polymerase II and U1 small nuclear ribonucleoprotein thereby coupling transcription and splicing. It binds also its own pre-mR and autoregulates its expression; this autoregulation mechanism is mediated by non-sense-mediated decay.
    FUS plays a role in D repair mechanisms by promoting D-loop formation and homologous recombination during D double-strand break repair. In neuronal cells, FUS plays crucial roles in dendritic spine formation and stability, R transport, mR stability and synaptic homeostasis.
  • Clinical and Translational Updates

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Price(EUR) : €350.00

Price(EUR) : €2330.00

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  • Number of Drugs in Clinical Trials:1 Details
  • Latest Research Phase:Phase 3 Clinical

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