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Your Position: Casa > Protein > IL-1 RAcP > ILP-HF223

FITC-Labeled Human IL-1 RAcP / IL-1 R3 Protein, His Tag

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  • Synonym
    IL1RAP,IL-1RAcP,C3orf13,IL1R3
  • Source
    FITC-Labeled Human IL-1 RAcP / IL-1 R3, His Tag(ILP-HF223) is expressed from human 293 cells (HEK293). It contains AA 21 Ser - 359 Glu (Accession # Q9NPH3-1).
    Predicted N-terminus: Ser 21
  • Molecular Characterization
    IL-1 RAcP Structure

    This protein carries a polyhistidine tag at the C-terminus

    The protein has a calculated MW of 39.69 kDa. The protein migrates as 50-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Conjugate

    FITC

    Excitation source: 488 nm spectral line, argon-ion laser

    Excitation Wavelength: 488 nm

    Emission Wavelength: 535 nm

  • Labeling
    The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with FITC using standard chemical labeling method. The residual FITC is removed by molecular sieve treatment during purification process.
  • Protein Ratio
    The FITC to protein molar ratio is 1.5-3.5.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please protect from light and avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
IL-1 RAcP SDS-PAGE

FITC-Labeled Human IL-1 RAcP / IL-1 R3, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
 IL-1 RAcP ELISA

Immobilized Human Anti-IL1RAP Human IgG1 at 1 μg/mL (100 μL/well) can bind FITC-Labeled Human IL-1 RAcP / IL-1 R3, His Tag (Cat. No. ILP-HF223) with a linear range of 0.002-0.313 μg/mL (QC tested).

  • Background
    Interleukin-1 receptor accessory protein (IL1RAP or IL-1RAcP) is also known as Interleukin-1 receptor 3 (IL-1R-3 or IL-1R3), C3orf13, which belongs to the interleukin-1 receptor family. IL1RAP contains three Ig-like C2-type (immunoglobulin-like) domains and one TIR domain. IL1RAP is detected in liver, skin, placenta, thymus and lung. IL-1R3 / IL1RAP is coreceptor with IL1R1, associates with IL1R1 bound to IL1B to form the high affinity interleukin-1 receptor complex which mediates interleukin-1-dependent activation of NF-kappa-B and other pathways.
  • Clinical and Translational Updates

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Price(EUR) : €320.00

Price(EUR) : €2200.00

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  • Latest Research Phase:Phase 2 Clinical

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