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The Human Th1/Th2 Cytokine Kit can be used to quantitatively measure Interleukin-2 (IL-2), Interleukin-4 (IL-4), Interleukin-6 (IL-6), Interleukin-10 (IL-10), Tumor Necrosis Factor-α (TNF-α), and Interferon-γ (IFN-γ) protein levels in a single sample.The kit performance has been optimized for analysis of specific cytokines in tissue culture supernatants, EDTA plasma, and serum samples.
It is for research use only.
| Assay Type | FACS |
| Analyte | IFN-γ, TNF-α, IL-10, IL-6, IL-4, IL-2 |
| Format | 96T |
| Reactivity | Human |
| Regulatory Status | RUO |
| Sensitivity | 1.77pg/ml (IFN-γ), 0.76pg/ml (TNF-α), 0.27pg/ml (IL-10), 0.78pg/ml ( IL-6), 0.65pg/ml (IL-4), 0.70pg/ml (IL-2) |
| Standard Curve Range | 9.8 ~ 10000pg/mL |
| Assay Time | 3 hr |
| Suitable Sample Type | human serum, human plasma, cell culture supernatants |
| Sample volume | 10 uL |
| ID | Components | Size |
| FCM05-C01 | Calibrator | 5ug（for each）×2 |
| FCM05-C02A | Human IL-2 Capture Beads | 96 tests |
| FCM05-C02B | Human IL-4 Capture Beads | 96 tests |
| FCM05-C02C | Human IL-6 Capture Beads | 96 tests |
| FCM05-C02D | Human IL-10 Capture Beads | 96 tests |
| FCM05-C02E | Human TNF-α Capture Beads | 96 tests |
| FCM05-C02F | Human IFN-γ Capture Beads | 96 tests |
| FCM05-C03 | 2 × Assay Buffer | 40 mL |
| FCM05-C04 | 10 × Wash Buffer | 10 mL |
| FCM05-C05 | Detection antibody | 0.5 mL |
| FCM05-C06 | 96-well V-bottom plate | 1 plate |
| FCM05-C07 | 96-well Sealing film | 2 pieces |
| FCM05-C08 | APC Positive Control | 0.5 mL |
| FCM05-C09 | PE Positive Control | 0.5 mL |
The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
A representative scatter plot showing single bead population in P1 gate (left), the beads conjugated with analyte-sepcific antibody differntiated by APC fluorescence intensity (middle), and fluorescence signal in propotion to concentration of the analyte was measured for each cytokine in PE channel (right). Data was generated on Beckman Cytoflex S Flow Cytometer, equipped with blue laser (488 nm) and red laser (640 nm).
A double logarithmic fitting curve was plotted for each cytokine( IL-2, IL-4, IL-6, IL-10, TNF-α, IFN-γ). The present curve of each cytokines was ranging from 9.8 pg/ml to 10000 pg/ml respectively .
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