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Thank you for choosing ACROBiosystems. Would you rate our product and service？Thank you for choosing ACROBiosystems. Would you rate our product and service？| Assay Type | Sandwich-ELISA |
| Analyte | IFN-γ |
| Format | 96T |
| Reactivity | Human |
| Regulatory Status | RUO |
| Sensitivity | 5.309 pg/mL |
| Standard Curve Range | 19.5 pg/mL-1250 pg/mL |
| Assay Time | 2 hr 50 min |
| Suitable Sample Type | For the quantitative determination of human IFN-γ in Cell Culture Supernatants, Plasma, Serum. |
| Sample volume | 100 μL |
| ID | Components | Size |
| CRS017-C01 | Pre-coated Anti-IFN-γ Antibody Microplate | 1 plate |
| CRS017-C02 | Human IFN-γ Standard | 30 μg |
| CRS017-C03 | Biotin-Anti-IFN-γ Antibody | 25 μg |
| CRS017-C04 | Streptavidin-HRP | 50 μL |
| CRS017-C05 | 10xWashing Buffer | 50 mL |
| CRS017-C06 | 2xDilution Buffer | 50 mL |
| CRS017-C07 | Substrate Solution | 12 mL |
| CRS017-C08 | Stop Solution | 7 mL |
A comprehensive validation of the ELISA method was performed following the ICH M10 on bioanalytical method validation and the FDA’s bioanalytical method validation guidance for industry. This validation included assessments of linearity, accuracy, precision, dilution linearity, recovery, and the hook effect. For details information, please refer to the DS.
ClinMax™ ELISA Kits are manufactured in a GMP-certified facility and comply to the ISO 13485 standard, ensuring a high level of quality and reliability.
The kit is developed for quantitative detection of IFN-γ in human serum and cell culture supernates.
It is for research use only.
The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
The kit shipped at room temperature that had been validated. Please contact us if you need blue ice shipping, but additional freight may be followed.
Below is the Shipping Statement for Kit Products.
Your experiment will include 6 simple steps:
a) Bring all reagents to room temperature(20°C-25°C) before use.
b) Add your sample to the plate and take the Human IFN-γ as standard. The samples and standard are diluted by Dilution Buffer.
c) Wash the plate and add the Biotin-Anti-IFN-γ Antibody diluted by Dilution Buffer to the plate.
d) Wash the plate and add the Streptavidin-HRP diluted by Dilution Buffer to the plate.
e) Wash the plate and add TMB.
f) Stop the substrate reaction by add diluted acid. Absorbance (OD) is calculated by the absorbance at 450 nm minus the absorbance at 630 nm to remove background disturbance before statistical analysis. The OD Value reflects the amount of bound protein.
For each experiment, each ELISA plate needs to set the standard curve. The following CRS-A017 standard curve data is for reference only.
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
Price(EUR) : €500.00
Price(EUR) :
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