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The kit is developed for quantitative detection of natural and recombinant human IFN-gamma in serum, plasma and cell culture supernatants.
It is suitable for potency assay of CAR-T/NK cell therapy.
It is for research use only.
| Analyte | IFN-γ |
| Assay Type | Sandwich-ELISA |
| Reactivity | Human |
| Sensitivity | 5.309 pg/mL |
| Range | 19.5 pg/mL-1250 pg/mL |
| Assay Time | 2 hr 50 min |
| Sample Type | Cell Culture Supernatants, Plasma, Serum. |
| Sample volume | 100 μL |
| Format | 96-wells plate breakable into 12 x 8 wells strips |
Elevate your research experience with our Cytokine/Biomarker Detection Kits, where accuracy, reliability, and ease of use are converging to deliver exceptional results.
For each experiment, a standard curve needs to be set for each microplate, and the specific OD value may vary depending on different laboratories, testers, or equipment. The following example data is for reference only. The sample concentration was calculated based on the results of the standard curve. The minimum detectable concentration of IFN-γ is less than 5.309 pg/mL.
Cell Culture Supernatant – Human peripheral blood mononuclear cells (PBMCs) were cultured and stimulated using anti-CD3&CD28 magnetic beads and several concentrations of lipopolysaccharide (LPS) then detected IFN-γ secretion amount on Day 3 (72 hours).
High concentrations of human IFN-γ were added to different diluted substrates for gradient dilution to evaluate the linearity of the assay.
20 replicates of each of 3 samples containing different IFN-γ concentrations were tested in one assay. Acceptable criteria: CV＜10%.
3 samples containing different concentrations of IFN-γ were tested in independent assays. Acceptable criteria: CV<10%.
IFN-γ was spiked into 3 human serum samples, and then analyzed. The average recovery of IFN-γ for serum samples is 93.5%, range is 85.2% - 105.2%.
| ID | Components | Size |
| CRS017-C01 | Pre-coated Anti-IFN-γ Antibody Microplate | 1 plate |
| CRS017-C02 | Human IFN-γ Standard | 30 μg |
| CRS017-C03 | Biotin-Anti-IFN-γ Antibody | 25 μg |
| CRS017-C04 | Streptavidin-HRP | 50 μL |
| CRS017-C05 | 10xWashing Buffer | 50 mL |
| CRS017-C06 | 2xDilution Buffer | 50 mL |
| CRS017-C07 | Substrate Solution | 12 mL |
| CRS017-C08 | Stop Solution | 7 mL |
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