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Your Position: Casa > Protein > Vinculin > VIN-H52H9

Human Vinculin / VCL Protein, His Tag

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  • Synonym
    Vinculin, VCL,MV,Metavinculin
  • Source
    Human Vinculin, His Tag(VIN-H52H9) is expressed from human 293 cells (HEK293). It contains AA Pro 2 - Gln 1066 (Accession # P18206-2).
    Predicted N-terminus: Pro 2
  • Molecular Characterization
    Vinculin Structure

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 118.5 kDa. The protein migrates as 130-145 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Vinculin SDS-PAGE

Human Vinculin, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
 Vinculin ELISA

Immobilized Human Vinculin, His Tag (Cat. No. VIN-H52H9) at 1 μg/mL (100 μL/well) can bind Vinculin Antibody with a linear range of 0.2-16 ng/mL (QC tested).

  • Background
    Vinculin is a ubiquitously expressed actin-binding protein and involves in cross-linking of the cell cytoskeleton with the extracellular matrix for mechanical support of the cell. Vinculin is frequently used as a marker for both cell-cell and cell-extracellular matrix (focal adhesion) adherens-type junctions.
  • Clinical and Translational Updates

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Price(EUR) : €350.00

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