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Your Position: Casa > Protein > IL-18 R1 > IL1-H82E7

Biotinylated Human IL-18 R1 / CD218a Protein, His,Avitag™ (MALS & SPR verified)

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  • Synonym
    IL18R1,IL1RRP,CD218a,IL18Ra
  • Source
    Biotinylated Human IL-18 R1, His,Avitag(IL1-H82E7) is expressed from human 293 cells (HEK293). It contains AA Ala 19 - Arg 329 (Accession # Q13478-1).
    Predicted N-terminus: Ala 19
  • Molecular Characterization
    IL-18 R1 Structure

    This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 39.3 kDa. The protein migrates as 55-65 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Labeling
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Protein Ratio
    Passed as determined by the HABA assay / binding ELISA.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
IL-18 R1 SDS-PAGE

Biotinylated Human IL-18 R1, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
IL-18 R1 MALS images

The purity of Biotinylated Human IL-18 R1, His,Avitag (Cat. No. IL1-H82E7) is more than 90% and the molecular weight of this protein is around 65-75 kDa verified by SEC-MALS.

Bioactivity-SPR
 IL-18 R1 SPR

Biotinylated Human IL-18 R1, His,Avitag (Cat. No. IL1-H82E7) captured on Biotin CAP-Series S Sensor Chip can bind Human IL-18, Tag Free with an affinity constant of 1.49 μM as determined in a SPR assay (Biacore 8K) (QC tested).

 IL-18 R1 SPR

Biotinylated Human IL-18 R1, His,Avitag (Cat. No. IL1-H82E7) captured on Biotin CAP-Series S Sensor Chip can bind Human IL-18, Tag Free (Premixed with Human IL-18 R beta / IL-1 R7, Fc Tag, Cat. No. ILB-H5256) with an affinity constant of 5.87 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).

  • Background
    Interleukin-18 receptor 1 (IL18R1) is also known as CD218 antigen-like family member A (CD218a), IL1 receptor-related protein (IL-1Rrp or IL1R-rp), CDw218a, IL18Ra, IL1RRP, which belongs to the interleukin-1 receptor family. IL18R1 contains three Ig-like C2-type (immunoglobulin-like) domains and one TIR domain. IL18R1 is receptor for interleukin 18 (IL-18). IL18R1 binds to the agonist leads to the activation of NF-kappa-B, but does not bind IL1A/interleukin-1 alpha or IL1B/interleukin-1 beta.
  • Clinical and Translational Updates

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