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Items | Specifications |
Material | Polystyrene |
Color | Clear |
Plate Blocking | 2% BSA Blocking Buffer |
Formulations | Clear, 96-well plates, coated with 100uL of Streptavidin and blocked with 200uL of 2% BSA Blocking Buffer, Dilution Buffer 50mL |
Detection Method | Colorimetric |
Capacity | ~5pmol biotin/well |
CV% of plates/wells | < 10% |
Type | Detection Plate, Immunoassay, ELISA |
Streptavidin (SA) has an extraordinarily high affinity for biotin with a dissociation constant (Kd) on the order of 10−14 mol/L, the Biotinylated molecules can bind to the SA irreversibly. Streptavidin has an isoelectric point of 5 to 6, resulting in low nonspecific interactions. The Streptavidin Coated Plates we provide are easy to use and widely available for applications. Example ELISA Procedure.
Immobilized Biotinylated SARS-CoV-2 S protein RBD, His, Avi tag at 1000 ng/mL (100 μL/well) on Streptavidin Coated Plates, Clear, 12×8-Well Strips, White Frame (For Serological Testing) (Cat. No. SP-13), can bind Anti-SARS-CoV antibody in serum with a linear range of 0.1-3 ng/mL (Routinely tested). The minimum OD is about 0.15.
Immobilized 0.1μg biotinylated protein on Streptavidin Coated Plates, Clear, 12×8-Well Strips, White Frame (For Serological Testing) (Cat. No. SP-13), the signal of detection antibody in serum were similar with other two plates which claim to reduce background.
There were no signal between detection antibody with no biotin in serum and Streptavidin Coated Plates, Clear, 12×8-Well Strips, White Frame (For Serological Testing) (Cat. No. SP-13), but competitors were positive with some antibodies.
Cat. No. SP-13, Adsorption stability test of avidin protein. After three SA plates were washed with buffer solution for three times, five times and ten times respectively, binding activity between the SA plate, the Spike RBD Protein and the Anti-SARS-CoV-2 Spike RBD Antibody showed no difference. The test shows that the SA protein has good stability on the plate.
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