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Your Position: Casa > Protein > NKp46 > NK6-H82E2

Biotinylated Human NKp46 / CD335 Protein, His Tag, ultra sensitivity (primary amine labeling)

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  • Synonym
    NCR1,LY94,CD335,NK-p46,hNKp46
  • Source
    Biotinylated Human NKp46 Protein, His Tag, primary amine labeling(NK6-H82E2) is expressed from human 293 cells (HEK293). It contains AA Gln 22 - Asn 254 (Accession # O76036-6).
    Predicted N-terminus: Gln 22
  • Molecular Characterization
    NKp46 Structure

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 28.3 kDa. The protein migrates as 35-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Labeling
    The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with biotins using standard chemical labeling method. A standard biotin reagent (13.5 angstroms) is used in this product.
  • Protein Ratio
    Passed as determined by the HABA assay / binding ELISA.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
NKp46 SDS-PAGE

Biotinylated Human NKp46 Protein, His Tag, primary amine labeling on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
 NKp46 ELISA

Immobilized Biotinylated Human NKp46 Protein, His Tag, primary amine labeling (Cat. No. NK6-H82E2) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Anti-NKP46 Antibody, Human IgG1 with a linear range of 0.1-1 ng/mL (QC tested).

 NKp46 ELISA

Immobilized Anti-NKP46 Antibody, Human IgG1 at 1 μg/mL (100 μL/well) can bind Biotinylated Human NKp46 Protein, His Tag, primary amine labeling (Cat. No. NK6-H82E2) with a linear range of 0.1-4 ng/mL (Routinely tested).

  • Background
    Natural cytotoxicity triggering receptor 1 (NCR1) is also known as Natural killer cell p46-related protein (NK-p46), Lymphocyte antigen 94 homolog (LY94), CD antigen CD335, which belongs to the natural cytotoxicity receptor (NCR) family. NCR1 contains two Ig-like (immunoglobulin-like) domains. NCR1 interacts with CD247 and FCER1G. NCR1 / CD335 may contribute to the increased efficiency of activated natural killer (NK) cells to mediate tumor cell lysis.
  • Clinical and Translational Updates

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  • Latest Research Phase:Phase 2 Clinical

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