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Human ITGAL&ITGB2 Heterodimer Protein, His Tag&Tag Free, produced by co-expression of ITGAL and ITGB2, has a calculated MW of 124.1 kDa (ITGAL) & 80.2 kDa (ITGB2). Subunit ITGAL is fused with an acidic tail at the C-terminus and followed by a polyhistidine tag and subunit ITGB2 contains no tag but a basic tail at the C-terminus. The protein migrates as 140-150 kDa (ITGAL) and 90-95 kDa (ITGB2) under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.
>95% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Human ITGAL&ITGB2 Heterodimer Protein, His Tag&Tag Free on SDS-PAGE under non-reducing (NR) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
Immobilized Human ITGAL&ITGB2 Heterodimer Protein, His Tag&Tag Free (Cat. No. IT2-H53W3) at 10 μg/mL (100 μL/well) can bind Human ICAM-1, Fc Tag (Cat. No. IC1-H5250) with a linear range of 0.4-6 ng/mL (QC tested).
Immobilized Human ICAM-1, Fc Tag (Cat. No. IC1-H5250) at 10 μg/mL (100 μL/well) can bind Human ITGAL&ITGB2 Heterodimer Protein, His Tag&Tag Free (Cat. No. IT2-H53W3) with a linear range of 0.156-5 μg/mL (Routinely tested).
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