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This protein carries a mouse IgG2a Fc tag at the C-terminus.
The protein has a calculated MW of 38.6 kDa. The protein migrates as 43-50 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Human CD3 epsilon, Mouse IgG2a Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of Human CD3 epsilon, Mouse IgG2a Fc Tag (Cat. No. CDN-H5253) is more than 90% and the molecular weight of this protein is around 75-90 kDa verified by SEC-MALS.
Immobilized Human CD3 epsilon, Mouse IgG2a Fc Tag (Cat. No. CDN-H5253) at 1 μg/mL (100 μL/well) can bind Mouse Anti-Human CD3 (SP34-2) (mouse IgG1) with a linear range of 9.8-39 ng/mL (QC tested).
Anti-Human CD3 mAb, mouse IgG1 (Clone # SP34-2) immobilized on CM5 Chip can bind Human CD3 epsilon, Mouse IgG2a Fc Tag (Cat. No. CDN-H5253) with an affinity constant of 75.2 nM as determined in a SPR assay (Biacore T200) (Routinely tested).
Bispecific T-cell Engager immobilized on CM5 Chip can bind Human CD3 epsilon, Mouse IgG2a Fc Tag (Cat. No. CDN-H5253) with an affinity constant of 20.1 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
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