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Limited Edition Golden Llama is here! Check out how you can get one. 
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This protein carries a polyhistidine tag at the N-terminus.
The protein has a calculated MW of 22.4 kDa. The protein migrates as 26 kDa and 28-30 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
>90% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Human TL1A, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
The purity of Human TL1A, His Tag (Cat. No. TLA-H5243) is more than 85% and the molecular weight of this protein is around 70-90 kDa verified by SEC-MALS.
Immobilized Human DcR3, Fc Tag (Cat. No. TNB-H5255) at 5 μg/mL (100 μL/well) can bind Human TL1A, His Tag (Cat. No. TLA-H5243) with a linear range of 0.3-2 ng/mL (QC tested).
Immobilized Human TL1A, His Tag (Cat. No. TLA-H5243) at 2 μg/mL (100 μL/well) can bind pre-mixed increasing concentrations of Monoclonal Anti-TL1A Antibody, Human IgG1 and 4 μg/mL (50 μL/well) Biotinylated Human DR3 Protein, Fc,Avitag (Cat. No. DR3-H82F3) with a half maximal inhibitory concentration (lC50) of 1.976 μg/mL (Routinely tested).
Immobilized Human TL1A, His Tag (Cat. No. TLA-H5243) at 10 μg/mL (100 μL/well) can bind Human DR3 Protein, Fc Tag (Cat. No. DR3-H5253) with a linear range of 0.005-0.156 μg/mL (Routinely tested).
Human DR3 Protein, His Tag (Cat. No. TN5-H52H3) immobilized on CM5 Chip can bind Human TL1A, His Tag (Cat. No. TLA-H5243) with an affinity constant of 45.8 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Anti-TL1A antibody captured on Protein A Chip can bind Human TL1A, His Tag (Cat. No. TLA-H5243) with an affinity constant of 0.765 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Human DcR3, Fc Tag (Cat. No. TNB-H5255) captured on Protein A Chip can bind Human TL1A, His Tag (Cat. No. TLA-H5243) with an affinity constant of 0.269 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Response to human TL1A protein (FOLD).
The DR3 (TL1A receptor) (Luc) Jurkat Reporter Cell was stimulated with serial dilutions of human TL1A protein (Cat.No.TLA-H5243). The max induction fold was approximately 40.12.
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