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This protein carries a polyhistidine tag at the C-terminus
The protein has a calculated MW of 26.6 kDa. The protein migrates as 33-35 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
SARS-CoV-2 S protein RBD (N501Y), His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of SARS-CoV-2 S protein RBD (N501Y), His Tag (Cat. No. SPD-C52Hn) is more than 90% and the molecular weight of this protein is around 28-38 kDa verified by SEC-MALS.
Immobilized Human ACE2, Fc Tag (Cat. No. AC2-H5257) at 1 μg/mL (100 μL/well) can bind SARS-CoV-2 S protein RBD (N501Y), His Tag (Cat. No. SPD-C52Hn) with a linear range of 2-39 ng/mL (QC tested).
Immobilized SARS-CoV-2 S protein RBD (N501Y), His Tag (Cat. No. SPD-C52Hn) at 1 μg/mL (100 μL/well) can bind Anti-SARS-CoV-2 RBD Potent Neutralizing Antibody, Human IgG1 (Cat. No. SPD-M180) with a linear range of 0.1-3 ng/mL (Routinely tested).
Serial dilutions of Monoclonal Anti-SARS-CoV-2 RBD Potent Neutralizing Antibody, Human IgG1 (Cat. No. SPD-M180) were added into SARS-CoV-2 S protein RBD (N501Y), His Tag (Cat. No. SPD-C52Hn) : Biotinylated Human ACE2, His,Avitag (Cat. No. AC2-H82E6) binding reactions. The half maximal inhibitory concentration (IC50) is 1.13169 (Routinely tested).
Human ACE2, Fc Tag (Cat. No. AC2-H5257) captured on CM5 chip via Anti-human IgG Fc antibodies surface can bind SARS-CoV-2 S protein RBD (N501Y), His Tag (Cat. No. SPD-C52Hn) with an affinity constant of 1.24 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
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