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Your Position: Casa > Protein > TSLP > TSP-C52H4

Cynomolgus TSLP (R127A, R130S) Protein, His Tag (HPLC verified)

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  • Synonym
    TSLP
  • Source
    Cynomolgus TSLP (R127A, R130S) Protein, His Tag(TSP-C52H4) is expressed from human 293 cells (HEK293). It contains AA Tyr 29 - Gln 159 (Accession # A0A7N9CAT7-1 (R127A, R130S)).
    Predicted N-terminus: Tyr 29
  • Molecular Characterization
    TSLP Structure

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 16.9 kDa. The protein migrates as 21-30 kDa under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

    >90% as determined by SEC-HPLC.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
TSLP SDS-PAGE

Cynomolgus TSLP (R127A, R130S) Protein, His Tag on SDS-PAGE under non-reducing (NR) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

SEC-HPLC
TSLP SEC-HPLC

The purity of Cynomolgus TSLP (R127A, R130S) Protein, His Tag (Cat. No. TSP-C52H4) was greater than 90% as determined by SEC-HPLC.

Bioactivity-ELISA
 TSLP ELISA

Immobilized Human TSLP R, Fc Tag (Cat. No. TSR-H525a) at 5 μg/mL (100 μL/well) can bind Cynomolgus TSLP (R127A, R130S), His Tag (Cat. No. TSP-C52H4) with a linear range of 1-4 ng/mL (QC tested).

 TSLP ELISA

Immobilized Cynomolgus TSLP (R127A, R130S), His Tag (Cat. No. TSP-C52H4) at 5 μg/mL (100 μL/well) can bind Monoclonal Anti-Human TSLP Antibody, Human IgG2 with a linear range of 0.5-8 ng/mL (Routinely tested).

  • Background
    Thymic stromal lymphopoietin (TSLP) is an epithelial cell-derived cytokine involved in the pathology of inflammatory skin diseases, and is widely expressed by epithelial cells. Human TSLP cDNA encodes a 159 amino acid (aa) residue precursor protein with a 28 aa signal sequence (4, 5). Human TSLP has been shown to developing nondeletional central tolerance, amplifying epithelium-induced class switching, inducing atopic diseases and maintaining intestinal noninflammatory environment. Among diverse cells responding to Human TSLP, CD11c+ dendritic cells are the most obviously characterized target cells.
  • Clinical and Translational Updates

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Price(EUR) : €350.00

Price(EUR) : €2570.00

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Drug Development Status

  • Number of Launched Drugs:1 Details
  • Number of Drugs in Clinical Trials:16 Details
  • Latest Research Phase:Approved

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