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Your Position: Casa > Protein > HMGB1 > HM1-H5255

Human HMGB1 Protein, Mouse IgG2a Fc Tag, low endotoxin

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  • Sinónimo
    HMGB1,HMG1,HMG3,SBP-1
  • Fuente
    Human HMGB1 Protein, Mouse IgG2a Fc Tag (HM1-H5255) is expressed from human 293 cells (HEK293). It contains AA Gly 2 - Glu 215 (Accession # P09429-1).
    Predicted N-terminus: Glu
  • Caracterización molecular
    Online(Gly 2 - Glu 215) P09429-1

    This protein carries a mouse IgG2a Fc tag at the N-terminus.

    The protein has a calculated MW of 51.6 kDa. The protein migrates as 60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxina
    Less than 0.1 EU per μg by the LAL method.
  • Pureza

    >95% as determined by SDS-PAGE.

  • Formulación

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitución

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Almacenamiento

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human HMGB1 Protein, Mouse IgG2a Fc Tag (Cat. No. HM1-H5255) SDS-PAGE gel

Human HMGB1 Protein, Mouse IgG2a Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-Cell based assay
Human FcRn Heterodimer Protein Cell_Base

Human HMGB1 Protein, Mouse IgG2a Fc Tag (Cat. No. HM1-H5255) stimulates production of TNF-α in Raw-246.7 cells. The EC50 for this effect is 13.18 μg/mL (Routinely tested).

  • Antecedentes
    High-mobility group protein B1 (HMGB1) is also known as high-mobility group protein 1 (HMG-1) and amphoterin, is a member of the HMGB family consisting of three members, HMGB1, HMGB2 and HMGB3. HMGB1 is a non-histone architectural chromosomal protein ubiquitously present in all vertebrate nuclei and binds double-stranded DNA without sequence specificity. The mechanism of inflammation and damage is binding to TLR4, which mediates HMGB1-dependent activation of macrophage cytokine release. This positions HMGB1 at the intersection of sterile and infectious inflammatory responses. HMGB1 has been studied as a DNA vaccine adjuvant and a target for cancer therapy.
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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ETA de productos en stock: 2 días laborables

Price(EUR) : €350.00

Price(EUR) : €2570.00

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  • Number of Drugs in Clinical Trials:2 Details
  • Latest Research Phase:Phase 1 Clinical

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