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This protein carries a human IgG1 Fc tag at the C-terminus
The protein has a calculated MW of 48.4 kDa. The protein migrates as 60-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Human IL-2 R alpha Protein, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of Human IL-2 R alpha Protein, Fc Tag (Cat. No. ILA-H5251) is more than 90% and the molecular weight of this protein is around 102-124 kDa verified by SEC-MALS.
Immobilized Human IL-2 R alpha Protein, Fc Tag (Cat. No. ILA-H5251) at 2 μg/mL (100 μL/well) can bind Human IL-2, Tag Free with a linear range of 0.4-3 ng/mL (QC tested).
Human IL-2 R alpha Protein, Fc Tag (Cat. No. ILA-H5251) captured on CM5 chip via anti-human IgG Fc antibody can bind Human IL-2, Tag Free with an affinity constant of 10.1 nM as determined in a SPR assay (Biacore T200) (Routinely tested)
Loaded Human IL-2 R alpha, Fc Tag (Cat. No. ILA-H5251) on Protein A Biosensor, can bind Human IL-2, Tag Free with an affinity constant of 43.2 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
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