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> Controles de isotipo de IgG
Los medicamentos con anticuerpos se han convertido en un campo importante en el desarrollo de la biomedicina. Los anticuerpos de control de isotipo en el desarrollo de fármacos de anticuerpos son de la misma clase y subtipo que el anticuerpo primario desarrollado contra el objetivo, pero no se unen específicamente a un objetivo conocido o potencial. El propósito de utilizar el control de isotipo es confirmar que la unión o el efecto del anticuerpo primario es específico, excluyendo las interacciones no específicas con las proteínas del receptor Fc o con otras proteínas. Mientras tanto, el control de isotipo también se puede utilizar como un anticuerpo que compite por la unión a las proteínas del receptor Fc, que está relacionado con el anticuerpo de bloqueo funcional que realiza la misma función, lo que facilita la evaluación efectiva y razonable de la eficacia de los medicamentos de anticuerpos. Los antecedentes inespecíficos son una variable importante que hay que controlar. El ajuste del anticuerpo de control de isotipo correspondiente para detectar la unión no específica puede reducir la generación de resultados falsos positivos y evaluar los posibles factores de influencia con precisión en el proceso de desarrollo de fármacos.
ACROBiosystems ha desarrollado una serie de controles de isotipos IgG con especies humanas, de ratón y de diferentes subtipos, que pueden ser utilizados como anticuerpos de control de isotipo en diversas aplicaciones como ELISA, western blot, inmunofluorescencia, inmunohistoquímica (IHC) y citometría de flujo (FACS), etc.
| Molecule | Cat. No. | Species | Product Description | Preorder/Order |
|---|
Human IgG1 (N297A) Kappa Isotype Control (mAb) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%. The purity of Human IgG1 (N297A) Kappa Isotype Control (mAb) (Cat. No. DNP-MB273) is more than 90% and the molecular weight of this protein is around 140-160 kDa verified by SEC-MALS.
Mouse IgG1 Kappa Isotype Control (mAb) on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%. The purity of Mouse IgG1 Kappa Isotype Control (mAb) (Cat. No. DNP-M1) is more than 90% and the molecular weight of this protein is around 130-160 kDa verified by SEC-MALS.
Immobilized DNP-Albumin Conjugate at 10 μg/mL (100 μL/well) can bind Human IgG1 Kappa Isotype Control (mAb) (Cat. No. DNP-M2) with a linear range of 1-10 ng/mL (QC tested).
Immobilized DNP-Albumin Conjugate at 10 μg/mL (100 μL/well) can bind Human IgG4 Kappa Isotype Control (mAb) (Cat. No. DNP-M3) with a linear range of 1-15 ng/mL (QC tested).
Immobilized DNP-Albumin Conjugate at 10 μg/mL (100 μL/well) can bind Human IgG1 (N297A) Kappa Isotype Control (mAb) (Cat. No. DNP-MB273) with a linear range of 1-10 ng/mL (QC tested).
Immobilized DNP-Albumin Conjugate, Bovine at 10 μg/mL (100 μL/well) can bind Mouse IgG1 Kappa Isotype Control (mAb) (Cat. No. DNP-M1) with a linear range of 2-20 ng/mL (QC tested).
2e5 of Anti-CD19 CAR-293 cells were stained with 100 μL of 1 μg/mL of Biotinylated Mouse IgG1 Isotype Control (mAb) (Cat. No. DNP-BM1) and positive control respectively, washed and then followed by PE-SA and analyzed with FACS (Routinely tested).
2e5 of anti-CD19 CAR-293 cells were stained with 100ul of 1:50 dilution of 2e5 of anti-CD19 CAR-293 cells were stained with 100ul of 1:50 dilution of Mouse IgG1 kappa Isotype Control (Cat. No. DNP-M1A1) and positive control antibody respectively, washed and then followed by PE anti-mouse IgG1 and analyzed with FACS (QC tested).
2e5 of anti-CD19 CAR-293 cells were stained with 100 μL of 1:50 dilution (2 μL stock solution in 100 μL FACS buffer) Biotinylated Mouse IgG1 kappa Isotype Control (Cat. No. DNP-BM190) and positive control antibody (Cat. No. FM3-BY54) respectively, washed and then followed by PE-SA and analyzed with FACS (QC tested).
2e5 of Anti-CD19 CAR-293 cells were stained with 100 μL of 1:50 dilution (2 μL stock solution in 100 μL FACS buffer) of FITC-Labeled Mouse IgG1 Isotype Control (Cat. No. DNP-FM1A1) and positive control (Cat. No. FM3-FY45P1) respectively, and FITC signal was used to evaluate the binding activity (QC tested).
5e5 of Anti-CD19 CAR-293 cells were stained with 100 μL of 1:50 dilution (2 μL stock solution in 100 μL FACS buffer) of PE-Labeled Monoclonal Mouse IgG1 Isotype Control (Cat. No. DNP-PM1) and positive control (Cat. No. FM3-HPY53) respectively, and PE signal was used to evaluate the binding activity (QC tested).
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