Eje de señalización TIGIT

TIGIT (inmunorreceptor de células T con dominios lg e ITIM) es una proteína del punto del control inmunitario recientemente descubierta que se expresa en las células T y en las células asesinas naturales (NK). TIGIT es un receptor inhibidor que modula la actividad inmunitaria compitiendo con el receptor activador CD226 (también conocido como DNAM-1) para la unión del ligando. Este interruptor de encendido y apagado de TIGIT y DNAM-1 es paralelo al de CTLA4 & CD28, lo que lo convierte en un objetivo prometedor para la terapia antitumoral.

CD155 (PVR) y CD112 (PVRL2 o Nectina-2) son co-ligandos de TIGIT y DNAM-1, mientras que CD113 (PVRL3 o Nectin-3) sólo se une a TIGIT. La afinidad entre los diferentes ligandos y los receptores varía. La unión entre TIGIT y CD155 es más robusta que la unión entre TIGIT y CD112 o CD113.

ACROBiosystems dispone de una amplia colección de proteínas recombinantes del eje de señalización TIGIT. Además de las proteínas etiquetadas con his y Fc comúnmente utilizadas, también disponemos de múltiples proteínas prebiotiniladas como TIGIT humana biotinilada, Avi Tag (Avitag™) (Cat. No. TIT-H82E5), proteína CD155 / PVR humana biotinilada, etiqueta Fc, etiqueta Avi (Avitag™) (Cat. No. CD5-H82F6) y la proteína Nectina-2 / CD112 humana biotinilada, etiqueta Fc, etiqueta Avi (Avitag™) (Cat. No. CD2-H82F8). A continuación encontrará información detallada:

Datos de bioactividad

Fig.1 FACS assay shows Biotinylated Human TIGIT, Fc,Avitag (Cat. No. TIT-H82F1) can bind to 293T cell overexpressing human CD155. The concentration of TIGIT is 0.03 μg/mL.

Protocol

Fig.2 FACS assay shows that Human TIGIT, Mouse IgG2a Fc tag, low endotoxin (Cat. No. TIT-H5253) can bind to 293T cell overexpressing human CD155. The concentration of TIGIT used is 0.03 μg/mL.

Protocol

Fig.3 Anti-Human TIGIT MAb (Mouse IgG1) captured on CM5 chip via Anti-Mouse antibodies surface, can bind Human TIGIT, His Tag (Cat. No. TIT-H52H3) with an affinity constant of 3.93 nM as determined in a SPR assay (Biacore T200).

Protocol

Fig.4 Serial dilutions of TIGIT antibody antibody (1:2 serial dilutions, from 20 μg/mL to 0.0097 μg/mL) were added into Human TIGIT, His Tag (Cat. No. TIT-H52H3): Biotinylated Human CD155, Fc Tag (Cat. No. CD5-H82F6) binding reactions. The assay was performed according to the above described protocol. Background was subtracted from data points before curve fitting.

Protocol

Lista de productos TIGIT

Cat. No.	Species	Product Description	Structure	Purity	Features
TIT-H82E5 (Biotin-labeled)		Biotinylated Human TIGIT Protein, Avi Tag
TIT-H82F1 (Biotin-labeled)		Biotinylated Human TIGIT Protein, Fc Tag, Avi Tag
TIT-C5223		Cynomolgus / Rhesus macaque TIGIT Protein
TIT-C5254		Cynomolgus / Rhesus macaque TIGIT Protein, Fc Tag
TIT-M52E6		Mouse TIGIT Protein, His Tag
TIT-M5257		Mouse TIGIT Protein, Fc Tag
TIT-R5258		Rat TIGIT Protein, Fc Tag
TIT-R5259		Rabbit TIGIT Protein, Fc Tag
TIT-H52H3		Human TIGIT Protein, His Tag
TIT-H5253		Human TIGIT Protein, Mouse IgG2a Fc Tag, low endotoxin
TIT-H5254		Human TIGIT Protein, Fc Tag
TIT-C52H7		Canine TIGIT Protein, His Tag

Otros productos relevantes

Molecule	Cat. No.	Species	Product Description	Structure	Purity	Features
CD155	CD5-H82F6		Biotinylated Human CD155 / PVR Protein, Fc, Avi Tag
	CD5-R52H5		Rhesus macaque CD155 / PVR Protein, His Tag
	CD5-H5254		Human CD155 / PVR Protein, Mouse IgG2a Fc Tag, low endotoxin
	CD5-M5254		Mouse CD155 / PVR Protein, Mouse IgG2a Fc Tag
	CD5-H82E3		Biotinylated Human CD155 / PVR Protein, His, Avitag™
	CD5-M82F7		Biotinylated Mouse CD155 / PVR Protein, Fc, Avitag™
	CD5-H5251		Human CD155 / PVR Protein, Fc Tag
	CD5-H5223		Human CD155 / PVR Protein, His Tag
CD112	CD2-H82F8		Biotinylated Human Nectin-2 / CD112 Protein, Fc, Avi Tag
	CD2-H5257		Human Nectin-2 / CD112 Protein, Mouse IgG2a Fc Tag
	PV2-H5253		Human Nectin-2 / CD112 Protein, Fc Tag
	PV2-H52E2		Human Nectin-2 / CD112 Protein, His Tag
CD113	PV3-H82F3		Biotinylated Human Nectin-3 / CD113 Protein, Fc, Avi Tag
	PV3-H5255		Human Nectin-3 / CD113 Protein, Fc Tag
	PV3-H52E4		Human Nectin-3 / CD113 Protein, His Tag
DNAM-1	DN1-H82F9		Biotinylated Human DNAM-1 / CD226 Protein, Fc, Avi Tag
	DN1-C52H9		Cynomolgus DNAM-1 / CD226 Protein, His Tag
	DN1-H5257		Human DNAM-1 / CD226 Protein, Fc Tag
	DN1-H52H6		Human DNAM-1 / CD226 Protein, His Tag

Comentarios de los clientes

Referencias

• Yu X, et al., 2009, Nat. Immunol. 10:48-57.

• Stengel KF, et al., 2012, Proc. Natl. Acad. Sci. U.S.A. 109:5399-5404.

• Lozano E, et al., 2012, J. Immunol. 188:3869-3875.

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