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A-Z
> TIGIT Signaling Axis
TIGIT (T cell immununo receptor with lg and ITIM domains) is a newly discovered immune checkpoint protein expressed on T cells and Natural Killer cells (NK). TIGIT is an inhibitory receptor which modulates the immune activity by competing with activating receptor CD226 (also known as DNAM-1) for ligand binding. This on and off switch of TIGIT & DNAM-1 parallels that of CTLA4 & CD28, which makes it a promising target for anti-tumor therapy.
CD155 (PVR) and CD112 (PVRL2 or Nectin-2) are co-ligands of TIGIT and DNAM-1, while CD113 (PVRL3 or Nectin-3) is only binding to TIGIT. The affinity between different ligands and receptors vary. The binding between TIGIT and CD155 is more robust than that between TIGIT and CD112 or CD113.
ACROBiosystems carries a large collection of recombinant proteins from TIGIT signaling axis. Please find detailed information below:
Fig.1 FACS assay shows Biotinylated Human TIGIT, Fc,Avitag (Cat. No. TIT-H82F1) can bind to 293T cell overexpressing human CD155. The concentration of TIGIT is 0.03 μg/mL.
Fig.2 FACS assay shows that Human TIGIT, Mouse IgG2a Fc tag, low endotoxin (Cat. No. TIT-H5253) can bind to 293T cell overexpressing human CD155. The concentration of TIGIT used is 0.03 μg/mL.
Fig.3 Anti-hTIGIT antibody captured on CM5 chip via Anti-mouse antibodies surface can bind Human TIGIT, His Tag (Cat. No. TIT-H52H5) with an affinity constant of 1.28 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Fig.4 Serial dilutions of Anti-TIGIT Neutralizing antibody (Catalog # EP131-C04) (1:1 serial dilution, from 5 μg/mL to 0.01μg/mL) was added into TIGIT: Biotinylated CD155 binding reactions. The assay was performed according to the protocol described below. Background was subtracted from data points prior to log transformation and curve fitting (QC tested).
| Cat. No. | Species | Product Description | Structure | Purity | Features |
|---|---|---|---|---|---|
|
TIT-H82F1 (Biotin-labeled) |
|
Biotinylated Human TIGIT Protein, Fc Tag, Avi Tag |
|
|
|
| TIT-C5223 |
|
Cynomolgus / Rhesus macaque TIGIT Protein |
|
|
|
| TIT-C5254 |
|
Cynomolgus / Rhesus macaque TIGIT Protein, Fc Tag |
|
|
|
| TIT-M52E6 |
|
Mouse TIGIT Protein, His Tag |
|
|
|
| TIT-M5257 |
|
Mouse TIGIT Protein, Fc Tag |
|
|
|
| TIT-R5258 |
|
Rat TIGIT Protein, Fc Tag |
|
|
|
| TIT-R5259 |
|
Rabbit TIGIT Protein, Fc Tag |
|
|
|
| TIT-H5253 |
|
Human TIGIT Protein, Mouse IgG2a Fc Tag, low endotoxin |
|
|
|
| TIT-H5254 |
|
Human TIGIT Protein, Fc Tag |
|
|
|
| TIT-C52H7 |
|
Canine TIGIT Protein, His Tag |
|
|
|
| Molecule | Cat. No. | Species | Product Description | Structure | Purity | Features |
|---|---|---|---|---|---|---|
| CD155 | CD5-H82F6 |
|
Biotinylated Human CD155 / PVR Protein, Fc, Avi Tag |
|
|
|
| CD5-R52H5 |
|
Rhesus macaque CD155 / PVR Protein, His Tag |
|
|
|
|
| CD5-H5254 |
|
Human CD155 / PVR Protein, Mouse IgG2a Fc Tag, low endotoxin |
|
|
|
|
| CD5-M5254 |
|
Mouse CD155 / PVR Protein, Mouse IgG2a Fc Tag |
|
|
|
|
| CD5-H82E3 |
|
Biotinylated Human CD155 / PVR Protein, His, Avitag™ |
|
|
|
|
| CD5-M82F7 |
|
Biotinylated Mouse CD155 / PVR Protein, Fc, Avitag™ |
|
|
|
|
| CD5-H5251 |
|
Human CD155 / PVR Protein, Fc Tag |
|
|
|
|
| CD5-H5223 |
|
Human CD155 / PVR Protein, His Tag |
|
|
||
| CD112 | CD2-H82F8 |
|
Biotinylated Human Nectin-2 / CD112 Protein, Fc, Avi Tag |
|
|
|
| CD2-H5257 |
|
Human Nectin-2 / CD112 Protein, Mouse IgG2a Fc Tag |
|
|
|
|
| PV2-H5253 |
|
Human Nectin-2 / CD112 Protein, Fc Tag |
|
|
|
|
| PV2-H52E2 |
|
Human Nectin-2 / CD112 Protein, His Tag |
|
|
|
|
| CD113 | PV3-H82F3 |
|
Biotinylated Human Nectin-3 / CD113 Protein, Fc, Avi Tag |
|
|
|
| PV3-H5255 |
|
Human Nectin-3 / CD113 Protein, Fc Tag |
|
|
|
|
| PV3-H52E4 |
|
Human Nectin-3 / CD113 Protein, His Tag |
|
|
|
|
| DNAM-1 | DN1-H82F9 |
|
Biotinylated Human DNAM-1 / CD226 Protein, Fc, Avi Tag |
|
|
|
| DN1-C52H9 |
|
Cynomolgus DNAM-1 / CD226 Protein, His Tag |
|
|
|
|
| DN1-H5257 |
|
Human DNAM-1 / CD226 Protein, Fc Tag |
|
|
|
|
| DN1-H52H6 |
|
Human DNAM-1 / CD226 Protein, His Tag |
|
|
|
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