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Your Position: Casa > Protein > Alpha-Synuclein > ALN-H5253

Human Alpha-Synuclein Protein, Fc Tag (MALS verified)

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  • Synonym
    SNCA,NACP,PARK1,alpha-Synuclein
  • Source
    Human Alpha-Synuclein, Fc Tag(ALN-H5253) is expressed from human 293 cells (HEK293). It contains AA Met 1 - Ala 140 (Accession # P37840-1).
    Predicted N-terminus: Met 1
  • Molecular Characterization
    Alpha-Synuclein Structure

    This protein carries a human IgG1 Fc tag at the C-terminus

    The protein has a calculated MW of 40.9 kDa. The protein migrates as 47-52 kDa and 53-56 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Alpha-Synuclein SDS-PAGE

Human Alpha-Synuclein, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
Alpha-Synuclein MALS images

The purity of Human Alpha-Synuclein, Fc Tag (Cat. No. ALN-H5253) is more than 90% and the molecular weight of this protein is around 75-95 kDa verified by SEC-MALS.

Bioactivity-ELISA
 Alpha-Synuclein ELISA

Immobilized Human Alpha-Synuclein, Fc Tag (Cat. No. ALN-H5253) at 1 μg/mL (100 μL/well) can bind Anti-Synucelin alpha/beta Monoclonal Antibody with a linear range of 0.031-1 μg/mL (QC tested).

  • Background
    Alpha-synuclein is a neuronal protein that plays several roles in synaptic activity such as regulation of synaptic vesicle trafficking and subsequent neurotransmitter release. It acts also as a molecular chaperone in its multimeric membrane-bound state, assisting in the folding of synaptic fusion components called SREs (Soluble NSF Attachment Protein REceptors) at presynaptic plasma membrane in conjunction with cysteine string protein-alpha/DJC5. Abnormalities in alpha-synuclein are implicated in the pathogenesis of Parkinson's disease (PD). Alpha-synuclein is present in Lewy-bodies, the neuropathological hallmark of PD, and the protein and its aggregation have been widely linked to neurotoxic pathways that ultimately lead to neurodegeneration.
  • Clinical and Translational Updates

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Price(EUR) : €160.00

Price(EUR) : €610.00

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Drug Development Status

  • Number of Launched Drugs:0 Details
  • Number of Drugs in Clinical Trials:20 Details
  • Latest Research Phase:Phase 3 Clinical

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