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Limited Edition Golden Llama is here! Check out how you can get one. 
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This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 65.1 kDa. The protein migrates as 75-95 kDa and >116 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
FITC
Excitation source: 488 nm spectral line, argon-ion laser
Excitation Wavelength: 488 nm
Emission Wavelength: 535 nm
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please protect from light and avoid repeated freeze-thaw cycles.
This product is stable after storage at:
FITC-Labeled Human CD5, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.
The purity of FITC-Labeled Human CD5, Fc Tag (Cat. No. CD5-HF255) is more than 90% and the molecular weight of this protein is around 135-165 kDa verified by SEC-MALS.
Immobilized FITC-Labeled Human CD5, Fc Tag (Cat. No. CD5-HF255) at 1 μg/mL (100 μL/well) can bind Mouse Anti-CD5 Antibody, Mouse IgG1 with a linear range of 0.2-3 ng/mL (QC tested).
5e5 of Anti-CD5 CAR-293 cells were stained with 100 μL of 3 μg/mL of FITC-Labeled Human CD5, Fc Tag (Cat. No. CD5-HF255) and negative control protein respectively. FITC signal was used to evaluate the binding activity (QC tested).
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