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AM359b
Human IgG1 | Human Kappa
Unconjugated
Recombinant Monoclonal
Virus
This product is a specific antibody against SARS-CoV-2 Spike protein RBD domain. Cross-reactivity with Spike protein RBD domain of other coronaviruses, including SARS-CoV, MERS-CoV, HCoV-229E, HCoV-NL63, HCoV-OC43 and HCoV-HKU1 has not been tested.
| Application | Recommended Usage |
| ELISA | 0.1-25 ng/mL |
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Protein A purified/ Protein G purified
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Anti-SARS-CoV-2 Spike RBD Broadly Neutralizing Antibody, Human IgG1 (AM359b) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
The purity of Anti-SARS-CoV-2 Spike RBD Broadly Neutralizing Antibody, Human IgG1 (AM359b) (Cat. No. SPD-M265) is more than 90% and the molecular weight of this protein is around 125-155 kDa verified by SEC-MALS.
Detection of Anti-SARS-CoV-2 Spike RBD Broadly Neutralizing Antibody, Human IgG1 (MALS verified) titer by compitive-ELISA Assay. Serial dilutions of Anti-SARS-CoV-2 Spike RBD Broadly Neutralizing Antibody, Human IgG1 (AM359b) (Cat. No. SPD-M265) were added into Human ACE2 / ACEH Protein, Fc Tag (Cat. No. AC2-H5257): HRP-SARS-CoV-2 Spike RBD, His Tag (B.1.1.529/Omicron) binding reactions. The half maximal inhibitory concentration (IC50) is 0.1578 μg/mL (QC tested).
Anti-SARS-CoV-2 Spike RBD Broadly Neutralizing Antibody, Human IgG1 (AM359b) (Cat.No. SPD-M265) neutralizes SARS-CoV-2 Spike RBD by inhibiting RBD: ACE2 interaction. The ACE2-coated plate is incubated with the wild type (WT) RBD or B.1.1.7, B.1.351, P.1, B.1.617.2, B.1.1.529 mutant and treated with the neutralizing antibody at increasing concentration. Percent inhibition is calculated based on the OD value.
Immobilized Anti-SARS-CoV-2 Spike RBD Broadly Neutralizing Antibody, Human IgG1 (AM359b) (Cat. No. SPD-M265) at 2 μg/mL, add increasing concentrations of SARS-CoV-2 Spike RBD, His Tag (BA.2/Omicron) (Cat. No. SPD-C522g) and SARS-CoV-2 Spike Trimer, His Tag (BA.4/Omicron) (Cat. No. SPN-C5229) and SARS-CoV-2 Spike Trimer, His Tag (BA.5/Omicron) (Cat. No. SPN-C522e), and then add Biotinylated Anti-SARS-CoV-2 Spike RBD Antibody, Human IgG1 (AM130) (Cat. No. S1N-M13L3) at 0.5 μg/mL. Detection was performed using HRP-conjugated streptavidin with sensitivity of 19.53 ng/mL (Routinely tested).
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