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| ID | Components | Size |
| EP115-C01 | Pre-coated Human ACE2 Microplate | 1 plate |
| EP115-C02 | SARS-CoV-2 Inhibitor | 20 μg |
| EP115-C03 | HRP-SARS-CoV-2 Spike RBD(B.1.1.529) | 15 μg |
| EP115-C04 | 10xWashing Buffer | 50 mL |
| EP115-C05 | Dilution Buffer | 50 mL |
| EP115-C06 | Substrate Solution | 12 mL |
| EP115-C07 | Stop Solution | 7 mL |
This kit is developed for screening inhibitors of SARS-CoV-2 (B.1.1.529).
It is for research use only.
2. Find the expiration date on the outside packaging and do not use reagents past their expiration date.
3. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
Your experiment will include 5 simple steps:
a) All reagents were returned to room temperature(20°C-25°C) before use.
b) Make series the tested sample and SARS-CoV-2 Inhibitor with ditlution buffer, HRP-SARS-CoV-2 RBD diluted with dilution buffer.
c) Add the diluted sample, Control and the HRP-SARS-CoV-2 RBD add the plate respectively.
d) Wash the plate and add TMB or other colorimetric HRP substrate.
e) Stop the substrate reaction by add diluted acid. Absorbance (OD) is calculated as the absorbance at 450 nm minus the absorbance at 630 nm to remove background prior to statistical analysis. The OD Value reflects the amount of antibody bound.
Incubation of SARS-CoV-2 inhibitor:Blocking the binding of HRP-SARS-CoV-2 Spike RBD(B.1.529) to Human ACE2.
Serial dilutions of SARS-CoV-2 inhibitor (Catalog # EP115-C02) (1:1 serial dilutions, from 25ug/mL to 0.049ug/mL(171.9nM to 0.34nM)) and loaded onto the plate coated by human ACE2 in the presence of HRP-SARS-CoV-2 Spike RBD(B.1.1.529). Background was subtracted from data points prior to log transformation and curve fitting.
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