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Your Position: Casa > Beads > Spike RBD > MBS-K037

SARS-CoV-2 (Delta) Spike RBD-coupled Magnetic Beads

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ItemsSize (2mg)Size(5mg X 2)
Particle size2 μm2 μm
Physical appearancePowder mixturePowder mixture
Amount of Coupled Protein≈1021 pmol (28.9 μg) SARS-CoV-2 (Delta) Spike RBD/mg beads≈1021 pmol (28.9 μg) SARS-CoV-2 (Delta) Spike RBD/mg beads
Binding Capacity>707 pmol (20μg) ACE2/mg beads>707 pmol (20μg) ACE2/mg beads
FormulationPBS, pH7.4, with 10% TrehalosePBS, pH7.4, with 10% Trehalose
Reconstitution2 mL sterile deionized water (1 mg beads/mL)5 mL sterile deionized water (1 mg beads/mL)
  • Background
    The SARS-CoV-2 (Delta) Spike RBD-coupled Magnetic Beads is produced by coupling biotinylated SARS-CoV-2 spike RBD to streptavidin-conjugated magnetic beads. The spike RBD coupled to the beads contains the critical mutations (L452R, T478K) identified in the SARS-CoV-2 Delta variants (also known as B.1.617.2). The pre-coupled beads are ready to use for capturing anti-SARS-CoV-2 antibody or ACE2 protein from your sample with high specificity.
  • Source
    SARS-CoV-2 (Delta) Spike RBD Protein is expressed from human 293 cells (HEK293). It contains AA Arg 319 - Lys 537(Accession # QHD43416.1 (L452R, T478K)).
  • Application
    This product is intended for immunocapture, biopanning and flow cytometry. This is a non-sterile product.
  • Reconstitution

    See Certificate of Analysis (CoA) for detailed instruction.

  • Storage
    Upon receipt, please store the beads at -20°C for 6 months in lyophilized state.Please avoid more than 3 freeze-thaw cycles once reconstitution, immediate use after reconstitution is highly recommended.
  • Assay Principles
    Antibody Purification: 1. Resuspend the lyophilized beads by adding the buffer of choice. 2. Add analyte to the suspension, mix and incubate to enable specific binding of the beads and the target protein. 3. Magnetize beads, remove supernatant, and wash unbound protein fractions to capture target protein-bound beads. 4. Wash, magnetize the beads and collect purified target protein for use in downstream applications.

    The magnetic beads technology makes use of the easy and efficient collection of beads in magnetic field to facilitate antibody purification in a simple workflow of “bind-wash-elute”. In contrast to common separation techniques, this method does not require columns or centrifugation, and is therefore ideal in high-throughput applications.

  • Formulation

    Please contact us for detailed information.

    Contact us for customized product form or formulation.

Typical Data
 Spike RBD TYPICAL DATA

Immobilized 28.9 μg SARS-CoV-2 (Delta) Spike RBD/1 mg beads can bind human ACE2 (Cat. No. AC2-H5257) with an EC50 of 1.198 μg/mL.

  • Clinical and Translational Updates

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Price(EUR) : €810.00

Price(EUR) : €2530.00

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