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Biotinylated Cynomolgus IL-2RB&IL-2RA&IL-2RG, Fc,Avitag&Fc,Avitag is produced by co-expression of IL-2RB and IL-2RA and IL-2RG, has a calculated MW of 53.0 kDa (IL-2RB) and 80.0 kDa (IL-2RA&IL-2RG). Subunit IL-2RB is fused with a human IgG1 Fc tag at the C-terminus, followed by a Avi tag (Avitag™) and subunit IL-2RA&IL-2RG is fused with a human IgG1 Fc tag at the C-terminus, followed by a Avi tag (Avitag™). The reducing (R) protein migrates as 65-70 kDa and 115-130 kDa respectively due to glycosylation.
>95% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Biotinylated Cynomolgus IL-2RB&IL-2RA&IL-2RG, Fc,Avitag&Fc,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of Biotinylated Cynomolgus IL-2RB&IL-2RA&IL-2RG, Fc,Avitag&Fc,Avitag (Cat. No. ILG-C82F6) is more than 85% and the molecular weight of this protein is around 200-240 kDa verified by SEC-MALS.
Immobilized Human IL-2, His Tag (Cat. No. IL2-H52H8) at 5 μg/mL (100 μL/well) can bind Biotinylated Cynomolgus IL-2RB&IL-2RA&IL-2RG, Fc,Avitag&Fc,Avitag (Cat. No. ILG-C82F6) with a linear range of 8-125 ng/mL (QC tested).
Loaded Biotinylated Cynomolgus IL-2RB&IL-2RA&IL-2RG, Fc,Avitag&Fc,Avitag (Cat. No. ILG-C82F6) on SA Biosensor, can bind Human IL-2, His Tag (Cat. No. IL2-H52H8) with an affinity constant of 0.755 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
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