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Biotinylated Human IL-2RB&IL-2RG Heterodimer Protein, Fc,Avitag&Fc,Avitag is produced by co-expression of IL-2RB and IL-2RG, has a calculated MW of 52.8 kDa (IL-2RB) and 55.3 kDa (IL-2RG). Subunit IL-2RB is fused with a human IgG1 Fc tag at the C-terminus, followed by an Avi tag (Avitag™) and subunit IL-2RG is fused with a human IgG1 Fc tag at the C-terminus, followed by an Avi tag (Avitag™). The protein migrates as 60-66 kDa and 80-90 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
>95% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Biotinylated Human IL-2RB&IL-2RG Heterodimer Protein, Fc,Avitag&Fc,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
The purity of Biotinylated Human IL-2RB&IL-2RG Heterodimer Protein, Fc,Avitag&Fc,Avitag (Cat. No. ILG-H82F3) is more than 85% and the molecular weight of this protein is around 140-155 kDa verified by SEC-MALS.
Immobilized Human IL-2, Tag Free at 5 μg/mL (100 μL/well) can bind Biotinylated Human IL-2RB&IL-2RG Heterodimer Protein, Fc,Avitag&Fc,Avitag (Cat. No. ILG-H82F3) with a linear range of 0.01-0.156 μg/mL (QC tested).
Immobilized Human IL-15, premium grade (Cat. No. IL5-H4117) at 5 μg/mL (100 μL/well) can bind Biotinylated Human IL-2RB&IL-2RG Heterodimer Protein, Fc,Avitag&Fc,Avitag (Cat. No. ILG-H82F3) with a linear range of 0.039-0.625 μg/mL (Routinely tested).
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