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Your Position: Casa > Protein > Spike NTD > S1D-C52E2

Biotinylated SARS-CoV-2 S1 protein NTD, His,Avitag™ (MALS verified)

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  • Synonym
    S1 protein NTD,Spike protein S1 NTD,BetaCoV S1-NTD
  • Source
    Biotinylated SARS-CoV-2 S1 protein NTD, His,Avitag(S1D-C52E2) is expressed from human 293 cells (HEK293). It contains AA Val 16 - Phe 318 (Accession # QHD43416.1).
    Predicted N-terminus: Val 16
  • Molecular Characterization
    Spike NTD Structure

    This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 38.0 kDa. The protein migrates as 53-90 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Labeling
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Protein Ratio
    Passed as determined by the HABA assay / binding ELISA.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Spike NTD SDS-PAGE

Biotinylated SARS-CoV-2 S1 protein NTD, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

SEC-MALS
Spike NTD MALS images

The purity of Biotinylated SARS-CoV-2 S1 protein NTD, His,Avitag (Cat. No. S1D-C52E2) is more than 90% and the molecular weight of this protein is around 55-76 kDa verified by SEC-MALS.

Bioactivity-ELISA
 Spike NTD ELISA

Immobilized Anti-SARS-CoV-2 Spike NTD Antibody, Chimeric mAb (Cat. No. SPD-M121) at 1 μg/mL (100 μL/well) can bind Biotinylated S1 protein NTD, His,Avitag (Cat. No. S1D-C52E2) with a linear range of 1-25 ng/mL (QC tested).

  • Background
    It's been reported that Coronavirus can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
  • Clinical and Translational Updates

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Price(EUR) : €280.00

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