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Your Position: Casa > Protein > CTLA-4 > CT4-M82E7

Biotinylated Mouse CTLA-4 Protein, His,Avitag™

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  • Synonym
    CTLA4,CD152
  • Source
    Biotinylated Mouse CTLA-4, His,Avitag(CT4-M82E7) is expressed from human 293 cells (HEK293). It contains AA Glu 36 - Phe 162 (Accession # P09793-1  ).
    Predicted N-terminus: Glu 36
  • Molecular Characterization
    CTLA-4 Structure

    This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™)

    The protein has a calculated MW of 17.6 kDa. The protein migrates as 27-33 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Labeling
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Protein Ratio
    Passed as determined by the HABA assay / binding ELISA.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
CTLA-4 SDS-PAGE

Biotinylated Mouse CTLA-4, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity-ELISA
 CTLA-4 ELISA

Immobilized Biotinylated Mouse CTLA-4, His,Avitag (Cat. No. CT4-M82E7) at 1 μg/mL (100 μL/well) on Streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate, can bind Ipilimumab with a linear range of 5-40 ng/mL (QC tested).

 CTLA-4 ELISA

Immobilized Mouse B7-1, Fc Tag (Cat. No. CD0-M5259) at 5 μg/mL (100 μL/well) can bind Biotinylated Mouse CTLA-4, His,Avitag (Cat. No. CT4-M82E7) with a linear range of 0.6-5 ng/mL (Routinely tested).

 CTLA-4 ELISA

Immobilized Mouse B7-2, Fc Tag (Cat. No. CD6-M5251) at 5 μg/mL (100 μL/well) can bind Biotinylated Mouse CTLA-4, His,Avitag (Cat. No. CT4-M82E7) with a linear range of 0.6-10 ng/mL (Routinely tested).

  • Background
    CTLA-4 (Cytotoxic T-Lymphocyte Antigen 4) is also known as CD152 (Cluster of differentiation 152), is a protein receptor that downregulates the immune system. CTLA4 is a member of the immunoglobulin superfamily, which is expressed on the surface of Helper T cells and transmits an inhibitory signal to T cells. The protein contains an extracellular V domain, a transmembrane domain, and a cytoplasmic tail. Alternate splice variants, encoding different isoforms. CTLA4 is similar to the T-cell co-stimulatory protein, CD28, and both molecules bind to CD80 and CD86, also called B7-1 and B7-2 respectively, on antigen-presenting cells. CTLA4 transmits an inhibitory signal to T cells, whereas CD28 transmits a stimulatory signal. Intracellular CTLA4 is also found in regulatory T cells and may be important to their function. Fusion proteins of CTLA4 and antibodies (CTLA4-Ig) have been used in clinical trials for rheumatoid arthritis.
  • Clinical and Translational Updates

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