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This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 23.1 kDa. The protein migrates as 35-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Rhesus macaque CD16, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.
The purity of Rhesus macaque CD16, His Tag (Cat. No. FC6-R52H6) is more than 90% and the molecular weight of this protein is around 40-50 kDa verified by SEC-MALS.
Rhesus macaque CD16, His Tag (Cat. No. FC6-R52H6) captured on CM5 Chip via anti-His antibody can bind Rituximab with an affinity constant of 0.232 μM as determined in SPR assay (Biacore T200) (QC tested).
Loaded Rhesus macaque CD16, His Tag (Cat. No. FC6-R52H6) on HIS1K Biosensor, can bind Rituximab with an affinity constant of 0.28 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
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