Glypican-3 (GPC3), a 70 kDa surface-bound proteoglycan, is an important biomarker of hepatocellular carcinoma (HCC) (Nakatsura et al., 2003). In the disease tissue, GPC3 is overexpressed at both gene and protein levels. It regulates multiple downstream signaling cascade, and by doing so stimulates macrophage engagement and promotes epithelial-mesenchymal transition (EMT). Therefore, GPC3 has been extensively investigated as a molecule of interest for molecular imaging and targeted therapy.

ACROBiosystems provides a comprehensive panel of human GPC3 proteins, including both His-tagged GPC3 (Cat. No. GP3-H5223) and Fc-fusion GPC3 (Cat. No. GP3-H5258). In addition, we also offer Cynomolgus GPC3 and Mouse GPC3 proteins in His-tagged and Fc-tagged format. The pre-biotinylated GPC3 protein (Avitag™) will come soon.

The interaction between GPC3 and growth factors (Zhou et al., 2017).

Human Glypican 3 / GPC3, Fc Tag, low endotoxin on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

Immobilized Human FGF basic, Tag Free (Cat. No. BFF-H4117) at 2 μg/mL (100 μL/well) can bind Human Glypican 3, Fc Tag (Cat. No. GP3-H5258) with a linear range of 0.48-7.8 ng/mL (QC tested).