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Monoclonal Anti-RSV-Pre-F0 specific Antibody, Mouse IgG1 (12C6)

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  • Source

    Monoclonal Anti-RSV-Pre-F0 specific Antibody, Mouse IgG1 (12C6) is a Mouse monoclonal antibody produced from a hybridoma created by fusing SP2/0 myeloma and Mouse B-lymphocytes.

  • Clone

    12C6

  • Species

    Mouse

  • Isotype

    Mouse IgG1 | Mouse Kappa

  • Conjugate

    Unconjugated

  • Antibody Type

    Hybridoma Monoclonal

  • Reactivity

    Virus

  • Immunogen

    Recombinant HRSV (A) Fusion glycoprotein F0 derived from human 293 cells.

  • Specificity

    This product is a specific antibody specifically reacts with Prefusion glycoprotein F0/pre-F protein (RSV).

  • Application
    ApplicationRecommended Usage
    ELISA0.2-100 ng/mL
  • Purity

    >95% as determined by SDS-PAGE.

  • Purification

    Protein A purified/ Protein G purified

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70 °C for 3 months after reconstitution;
SDS-PAGE
Prefusion glycoprotein F0/pre-F protein (RSV) SDS-PAGE

Monoclonal Anti-RSV-Pre-F0 specific Antibody, Mouse IgG1 (12C6) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
 Prefusion glycoprotein F0/pre-F protein (RSV) ELISA

Immobilized HRSV (A) Pre-fusion glycoprotein F0, His Tag (MALS verified) (Cat. No. RSF-V52H7) at 2 μg/mL (100 μL/well) can bind Monoclonal Anti-RSV-Pre-F0 specific Antibody, Mouse IgG1 (12C6) (Cat. No. RS0-Y132) with a linear range of 0.098-3.125 ng/mL. The antibody does not bind HRSV (A) Post-fusion glycoprotein F0, His Tag (MALS verified) (Cat. No. RSF-V52H6) (QC tested).

 Prefusion glycoprotein F0/pre-F protein (RSV) ELISA

Immobilized HRSV (B) Pre-fusion glycoprotein F0, His Tag (Cat. No. RSF-V52H8) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-RSV-Pre-F0 specific Antibody, Mouse IgG1 (12C6) (Cat. No. RS0-Y132) with a linear range of 0.5-8 ng/mL. HRSV (B) Post-fusion glycoprotein F0, His Tag (Cat. No. RSF-V52H9) is verified not recoginized by Monoclonal Anti-RSV-Pre-F0 specific Antibody, Mouse IgG1 (12C6) (Cat. No. RS0-Y132) (Routinely tested).

  • Background
    Human respiratory syncytial virus (HRSV) is the most common etiological agent of acute lower respiratory tract disease in infants and can cause repeated infections throughout life. The RSV fusion glycoprotein (RSV F) is the principal target of RSV neutralizing antibodies in human sera. The RSV F is a type I viral fusion protein synthesized as inactive, single-chain polypeptides that assemble into trimers. RSV F fuses the viral and host cell membranes by irreversible protein refolding from the labile prefusion conformation to the stable post-fusion conformation.
  • Clinical and Translational Updates

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Price(EUR) : €350.00

Price(EUR) : €2330.00

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