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Your Position: Casa > Protein > CAS9 > CA9-S5149

GENPower™ NLS-Cas9 Nuclease, premium grade

GMP version GMP-CA9S18 is now available for seamless transition.

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  • Features

    High cleavage activity in different cell line
    High purity (>95% SDS-PAGE / SEC-MALS)
    Animal-Free materials
    Batch-to-batch consistency
    Stringent quality control tests

  • Product Details
    GENPower™ NLS-Cas9 Nuclease is a recombinant Streptococcus pyogenes Cas9 protein purified from Escherichia coli for CRISPR-based genome editing. The introduction of nuclear localization signals (NLS) can help Cas9 enter the nucleus, increasing the chance of genomic DNA cleavage.
  • Concentration
    10 mg/mL
  • Purity

    >95% as determined by SDS-PAGE.

    >95% as determined by SEC-MALS.

  • Host Cell Protein
    ≤10 ng/mg of protein tested by ELISA.
  • Host Cell DNA
    ≤1 ng/mg of protein tested by qPCR.
  • Sterility
    Negative
  • Mycoplasma
    Negative.
  • Endotoxin
    Less than 0.01 EU per μg by the LAL method.
  • Formulation

    Supplied as 0.2 μm filtered solution in 20 mM Tris, 300 mM NaCl, 0.1 mM EDTA, pH7.5 with glycerol as protectant.

    Contact us for customized product form or formulation.

  • Shipping

    This product is supplied and shipped with dry ice, please inquire the shipping cost.

  • Storage

    This product is stable after storage at:

    1. The product MUST be stored at -20°C or lower upon receipt;
    2. -20°C for 18 months under sterile conditions.
SDS-PAGE
CAS9 SDS-PAGE

The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

SEC-MALS
CAS9 MALS images

The purity of GENPower™ NLS-Cas9 Nuclease, premium grade (Cat. No. CA9-S5149) is more than 95% and the molecular weight of this protein is around 140-180 kDa verified by SEC-MALS.

Bioactivity
 CAS9 ENZYME

GENPower™ NLS-Cas9 Nuclease, premium grade is evaluated in an in vitro DNA cleavage assay on a DNA fragment containing the target sequence. The activity of the GENPower™ NLS-Cas9 Nuclease, premium grade is greater than 90% (QC tested).

 CAS9 ENZYME

The cleavage activity of GENPower™ NLS-Cas9 Nuclease, premium grade in vitro.

 CAS9 ENZYME

The cleavage activity of GENPower™ NLS-Cas9 Nuclease, premium grade in cell line.

 CAS9 ENZYME

The cleavage activity of GENPower™ NLS-Cas9 Nuclease, premium grade in human primary T cells.

 CAS9 ENZYME

The knockout efficiency for B2M in primary T cell was measured by Flow Cytometry.

 CAS9 ENZYME

The knockout efficiency for TRAC in Jurkat cell was measured by Flow Cytometry.

 CAS9 ENZYME

The knockout efficiency for B2M in Jurkat cell was measured by Flow Cytometry.

Bioactivity-Stability
 CAS9 STABILITY

The bioactivity based assay shows batch-to-batch consistency between Acro's GMP and PG Cas9.

  • Clinical and Translational Updates

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